Syn-PER™ 突触蛋白提取试剂
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Syn-PER™ 突触蛋白提取试剂
Thermo Scientific™

Syn-PER™ 突触蛋白提取试剂

Thermo Scientific Syn-PER 突触蛋白提取试剂是一种专有的细胞裂解试剂,用于从神经元组织和原代培养神经元中高效分离含功能性突触蛋白的突触体。Syn-PER 突触蛋白提取试剂的特点:•高效提取突触—获得高达 10 µg 突触蛋白/毫克神经元组织或 4了解更多信息
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货号数量
87793100 mL
货号 87793
价格(CNY)
3,458.00
Each
添加至购物车
数量:
100 mL
请求批量或定制报价
价格(CNY)
3,458.00
Each
添加至购物车
Thermo Scientific Syn-PER 突触蛋白提取试剂是一种专有的细胞裂解试剂,用于从神经元组织和原代培养神经元中高效分离含功能性突触蛋白的突触体。

Syn-PER 突触蛋白提取试剂的特点:

高效提取突触—获得高达 10 µg 突触蛋白/毫克神经元组织或 4 µg 突触蛋白/35 mm 培养皿原代培养神经元(106 个细胞)
温和配方—可分离活的突触体;然后裂解这些突触以提取天然突触蛋白并保持磷蛋白完整性
快速程序—在不到一小时内获得完整突触的突触体悬浮液
简单的方案—无需超速离心步骤

Syn-PER 突触蛋白提取试剂的应用:
•分离功能性突触体以研究神经递质释放
•提取突触前和突触后蛋白,以识别蛋白组成和突触功能的变化
•保存并研究与突触相关的不稳定或短暂的蛋白磷酸化事件

Syn-PER 试剂用于制备含具生物学活性的突触前和突触后蛋白的突触体(即完整的膜和突触蛋白复合物)。当与新鲜神经元组织或原代培养神经元配合使用时,使用 Syn-PER 试剂制备的突触体可用于研究突触传递。这些提取物中包含的突触体蛋白也可用于下游应用,如 Western 印迹、免疫沉淀、酶活性测定和蛋白-蛋白相互作用研究。Syn-PER 突触蛋白提取试剂可尽可能减少磷蛋白降解,适用于需要保持磷蛋白完整性的研究。

Syn-PER 突触蛋白提取试剂可高效富集突触体内的突触前和突触后蛋白,且得率较高。非变性细胞裂解试剂可与许多下游应用兼容,包括神经递质释放测定、酶测定(例如,磷酸酶、激酶)、免疫检测、各种色谱程序和电泳。此外,即使在不存在磷酸酶抑制剂的情况下,与大多数市售提取缓冲液相比,Syn-PER 试剂也可更好地保持磷蛋白完整性。Syn-PER 试剂不含蛋白酶或磷酸酶抑制剂。然而,抑制剂(如 Thermo Scientific Halt 蛋白酶和/或磷酸酶抑制剂)可在临用前添加,以防止蛋白水解或针对脑组织中通常存在的高磷酸酶活性提供额外保护。

突触体是在神经组织均质化过程中产生的孤立神经末梢。突触体制剂可用于提取突触前和突触后蛋白,因为其含有完整的突触前末梢(包括线粒体和突触囊泡)以及突触后膜和突触后致密区。突触体通常用于研究突触功能,因为其含有功能性离子通道、受体、酶和完整的突触囊泡,可吸收和释放神经递质。突触体制备通常涉及多个超速离心步骤和密度梯度,以将该细胞部分与其他细胞器分离。

更多产品数据
用于分离功能性突触体的方法

相关产品
N-PER™ 神经元蛋白提取试剂
仅供科研使用。不可用于诊断程序。
规格
产品规格液体
数量100 mL
容积(公制)100 mL
产品线Syn-PER™
产品类型试剂名称
Unit SizeEach
内容与储存
接收后,请将产品储存在 4°C 下。

常见问题解答 (FAQ)

Will yield be the same with cultured neurons when using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793), as compared to using fresh tissue?

We can not guarantee that the total synaptic yield from cultured neurons should be comparable to using fresh tissue.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Will yield be the same if I pellet and freeze neurons in culture when using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793), as compared to using fresh tissue?

We have tested using Syn-PER Synaptic Protein Extraction Reagent with frozen (mouse) brain tissue and total synaptic protein yields were comparable to fresh brain tissue.

Please note, frozen tissues are not recommended for synaptosome function tests such as neurotransmitter release and up take assays because synaptosomes from frozen tissues are not viable. This is a difference between frozen and fresh tissue in terms of application.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I perform RNA extraction after using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793)?

Yes. RNA extraction can be performed after using Syn-PER Synaptic Protein Extraction Reagent (Cat. No. 87793). After the lysis step, you will need to perform two centrifugations (step 4 and 5 in the manual, the first at low speed to discard the tissue or cell debris. The second one, at higher speed, will pellet synaptosomes. The supernatant arising from this step will include the cytosolic fraction (step 6).

If the synaptosomes are intact and have RNA inside, the RNA should be extractable by the kit or method of your choice.

Please note: This extraction procedure should be experimentally tested.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Can you provide the shelf-life for the Syn-PER Synaptic Protein Extraction Reagent?

The Syn-PER Synaptic Protein Extraction Reagent is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.