PrimeFlow™microRNA 预处理缓冲液

PrimeFlow™ microRNA 预处理缓冲液设计用于与 PrimeFlow™ RNA 检测（货号 88-18005-204）结合用于通过流式细胞术对 microRNA了解更多信息

货号	数量
88-18006	100 次测试

货号 88-18006

价格（CNY)

5,526.00

添加至购物车

100 次测试

价格（CNY)

5,526.00

添加至购物车

PrimeFlow™ microRNA 预处理缓冲液设计用于与 PrimeFlow™ RNA 检测（货号 88-18005-204）结合用于通过流式细胞术对 microRNA 和其他小 RNA 进行较佳检测。由于 microRNA 分子的每个分子的靶标探针结合位点数量有限，我们建议使用 1 型（Alexa Fluor™ 647）或 10 型（Alexa Fluor™ 568 靶标探针组）以实现较佳灵敏度。本缓冲液也可与 PrimeFlow™ Fixation/Permeabilization Buffer Set（货号 88-17000-210）结合使用以评价细胞内靶标的抗体性能。遵循随附于此数据表的方案。请注意，PrimeFlow RNA 检测杂交步骤后，可能会观察到抗体性能的其他变化。

适用于目标基因的 PrimeFlow™ RNA 检测和靶标探针组单独出售。

仅供科研使用。不可用于诊断程序。

形式Liquid

产品类型microRNA 预处理缓冲液

数量100 次测试

Unit SizeEach

内容与储存

PrimeFlow™ microRNA 预处理浓缩液 (4X)：货号。00-16009，25 mL，在 2-8°C 下储存
PrimeFlow™ microRNA 预处理稀释液：货号。00-16008，75 mL，在 2-8°C 下储存

常见问题解答 (FAQ)

What is the sensitivity for microRNA detection with the PrimeFlow RNA Assay kit with the miRNA Pretreatment Buffrer (Cat. No 88-18006)?

The sensitivity is estimated to be 40 copies per cell with Type 1, Alexa Fluor 647 Probe Sets. The actual sensitivity may vary depending on the specific target and Probe Type.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the microRNA Pretreatment buffer (Cat. No. 88-18006) required for microRNA detection in the PrimeFlow RNA Assay?

The Pretreatment Buffer is not absolutely required for microRNA detection. However, it can enhance detection for most of the microRNA targets tested.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Will there be an expiration date on the PrimeFlow microRNA Pretreatment Buffer bottle label? If not, what is the shelf-life?

Expiration dates are printed on the labels of each component.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are tandem dyes affected by the PrimeFlow microRNA Pretreatment Buffer?

No changes for tandem dyes have been observed with the PrimeFlow microRNA Pretreatment Buffer (Cat. No 88-18006).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

In the table for performance of intracellular antibodies (Table 1, PrimeFlow microRNA Pretreatment Buffer user manual), does this mean all formats of the clones listed with a negative are not compatible with the PrimeFlow RNA Assay?

In the case of antibodies for Foxp3, we believe the clones do not stain after the pretreatment step, so we do not expect any formats for those clones to work.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all PrimeFlow™microRNA 预处理缓冲液 FAQs