ViewRNA™ Cell Plus 检测试剂盒

引用和文献 (10)

Invitrogen™

ViewRNA™ Cell Plus 检测试剂盒

ViewRNA Cell Plus 检测是一种结合了免疫细胞化学技术与 ViewRNA 技术（一种专有的荧光原位杂交和连续分支 DNA 扩增技术）的新型检测，可对单个细胞中的具有单分子灵敏度的了解更多信息

货号	数量
88-19000-99	1 kit

货号 88-19000-99

价格（CNY)

25,983.00

添加至购物车

1 kit

价格（CNY)

25,983.00

添加至购物车

ViewRNA Cell Plus 检测是一种结合了免疫细胞化学技术与 ViewRNA 技术（一种专有的荧光原位杂交和连续分支 DNA 扩增技术）的新型检测，可对单个细胞中的具有单分子灵敏度的 RNA 和蛋白进行可视化。本检测方法支持使用间接和直接免疫细胞化学技术同时检测多达三种 RNA 靶标，从而实现对细胞表面或细胞内蛋白的免疫表型分析，适用于对特定细胞亚群进行详细表征。用于同时检测第四个 RNA 靶标，ViewRNA ISH 细胞 740 模块（货号 QVC0200）可与本试剂盒配合使用。可分析 740 通道 (AlexaFluor 750) 的额外靶标。

ViewRNA Cell Plus 检测试剂盒含有进行检测所需的所有试剂。目标基因及抗体的靶标探针组单独出售。

反应性/物种
探针可设计用于任何物种。这些是较常用/检测的物种：亚美尼亚仓鼠、狒狒、牛、犬、猫、鸡、黑猩猩、奶牛、食蟹猴、驴、山羊、金黄叙利亚仓鼠、豚鼠、仓鼠、马、人、猕猴、猴、小鼠、非人灵长类动物、橄榄狒狒、猪、豚尾猴、兔、大鼠、恒河猴、绵羊

已报告应用
显微镜检查、免疫细胞化学技术

仅供科研使用。不可用于诊断程序。

适用于（应用）显微镜检查、免疫细胞化学技术

产品类型RNA 原位杂交测定试剂盒

数量1 kit

检测方法引物-探针检测

产品规格试剂盒

Unit SizeEach

常见问题解答 (FAQ)

How do ViewRNA assays compare to RNAScope assays?

ViewRNA and RNAScope technologies rely on the same signal amplification strategy - branched DNA amplification. Historically, both ViewRNA and RNAScope technologies originated from the same company, Panomics. The assays are expected to yield similar sensitivity and resolution, however each technology relies on its own set of proprietary reagents and probe set designs. Hence, the assays are not considered interchangeable or compatible. ViewRNA probe sets are not tested for RNAScope assaya and vice versa.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is the signal amplified in ViewRNA assays?

The ViewRNA technology relies on branched DNA signal amplification strategy. Target probes complementary to the target transcript sequence are further hybridized with pre-amplifier, amplifier and label probes that consist of branched DNA, and form 'tree branches' that allow numerous label probes to attach. This approach allows higher signal amplification compared to traditional ISH techniques.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find general information about ViewRNA ISH Assays?

For general information about ViewRNA ISH Assays, please go to this page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For ViewRNA assays, can I use the same set of wash solutions for all samples, including the positive and negative controls?

We do not recommend doing this. The negative control should be processed and washed separately from the rest of the samples. This is because the negative control does not contain any target probe sets and only the amplification reagents are added to it. If experimental samples are washed in the same beaker of wash solutions as the negative control, any unbound target probes that wash away can carry over to the negative control sample and cause unexpected positive signal (that will also appear to be very specific).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

To perform ViewRNA assays, which incubator oven should I use, Cat. No. QS0704 or QS0712?

Cat. No. QS0704 is a 120 V unit, for use in US/Canada region (https://www.thermofisher.com/order/catalog/product/QS0704).

Cat. No. QS0712 is a 220 V unit, for use in European region (https://www.thermofisher.com/order/catalog/product/QS0712).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all ViewRNA™ Cell Plus 检测试剂盒 FAQs

引用和文献 (10)

引用和文献

Abstract

Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes.

Authors:Rheaume BA, Jereen A, Bolisetty M, Sajid MS, Yang Y, Renna K, Sun L, Robson P, Trakhtenberg EF

Journal:Nat Commun

PubMed ID:30018341

'Retinal ganglion cells (RGCs) convey the major output of information collected from the eye to the brain. Thirty subtypes of RGCs have been identified to date. Here, we analyze 6225 RGCs (average of 5000 genes per cell) from right and left eyes by single-cell RNA-seq and classify them into 40 ... More

miR-103 promotes endothelial maladaptation by targeting lncWDR59.

Authors:Natarelli L, Geißler C, Csaba G, Wei Y, Zhu M, di Francesco A, Hartmann P, Zimmer R, Schober A

Journal:Nat Commun

PubMed ID:29980665

'Blood flow at arterial bifurcations and curvatures is naturally disturbed. Endothelial cells (ECs) fail to adapt to disturbed flow, which transcriptionally direct ECs toward a maladapted phenotype, characterized by chronic regeneration of injured ECs. MicroRNAs (miRNAs) can regulate EC maladaptation through targeting of protein-coding RNAs. However, long noncoding RNAs (lncRNAs), ... More

Long non-coding RNAs influence the transcriptome in pulmonary arterial hypertension: the role of PAXIP1-AS1.

Authors:Jandl K, Thekkekara Puthenparampil H, Marsh LM, Hoffmann J, Wilhelm J, Veith C, Sinn K, Klepetko W, Olschewski H, Olschewski A, Brock M, Kwapiszewska G

Journal:J Pathol

PubMed ID:30450722

'In idiopathic pulmonary arterial hypertension (IPAH), global transcriptional changes induce a smooth muscle cell phenotype characterised by excessive proliferation, migration, and apoptosis resistance. Long non-coding RNAs (lncRNAs) are key regulators of cellular function. Using a compartment-specific transcriptional profiling approach, we sought to investigate the link between transcriptional reprogramming by lncRNAs ... More

Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry.

Authors:van Buuren N, Kirkegaard K

Journal:Bio Protoc

PubMed ID:30505886

Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial world. We have previously discovered that higher barriers to the development of drug resistance can ... More

KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system.

Authors:Yang L, Song L, Liu X, Bai L, Li G

Journal:EMBO Rep

PubMed ID:30389724

Despite the success of animal cloning by somatic cell nuclear transfer (SCNT) in many species, the method is limited by its low efficiency. After zygotic genome activation (ZGA) during mouse development, a large number of endogenous retroviruses (ERVs) are expressed, including the murine endogenous retrovirus-L (MuERVL/MERVL). In this study, we ... More

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