HisPur™ Ni-NTA 磁珠
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
HisPur™ Ni-NTA 磁珠
引用和文献 (3)
Thermo Scientific™

HisPur™ Ni-NTA 磁珠

Thermo Scientific HisPur Ni-NTA 磁珠为高容量镍-IMAC 微珠，用于亲和纯化 His 标签融合蛋白（手动或自动模式）了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
8883210 mL
888312 mL
货号 88832
价格（CNY)
11,065.00
Each
添加至购物车
数量:
10 mL
请求批量或定制报价
价格（CNY)
11,065.00
Each
添加至购物车
Thermo Scientific HisPur Ni-NTA 磁珠为高容量镍-IMAC 微珠，用于亲和纯化 His 标签融合蛋白（手动或自动模式）。

HisPur Ni-NTA 磁珠的特点：

•高容量— 结合容量与其他供应商的 Ni-NTA 磁珠相当或高于后者
•低非特异性结合— 对微珠表面进行预封闭，方案提供经优化的缓冲液用于纯化
•快速— 方案可在 1 小时内完成
•可扩展— 可处理微升至毫升级的样品体积
•通用— 可在天然或变性条件下纯化蛋白
•试剂兼容— 可与常见细胞裂解试剂和各种缓冲液添加剂配合使用
•多种规格— 微珠偶联蛋白和下游应用可通过手动方式或在自动化平台（例如，Thermo Scientific KingFisher 仪器）上进行

封闭磁珠表面使用次氮基三乙酸 (NTA) 螯合基团进行衍生化，并带有二价镍离子 (Ni2+)。固定化金属亲和色谱 (IMAC) 微珠可提供高结合容量和极低的背景。HisPur Ni-NTA 磁珠可与磁力架配合用于手动操作，亦可用于自动化平台，如 Thermo Scientific KingFisher 仪器，以适应高通量需求。

HisPur Ni-NTA 用于重组 His 标签蛋白的小规模亲和纯化和高通量筛选。多聚组氨酸标签是极为常用的亲和标签，通常由 6 个连续组氨酸残基 (6xHis) 组成。这些标签蛋白在许多不同系统（极常见于细菌）中过表达，纯化自细胞裂解物（如使用 B-PER 细菌蛋白提取试剂所制备的细胞裂解物）。采用带有镍离子的 NTA 螯合物（与组氨酸侧链相匹配）对 His 标签蛋白进行纯化。NTA 螯合物含有四个金属结合位点，可提供低金属离子浸出率和高结合容量。HisPur Ni-NTA 磁珠的实验方案已经过优化，以提供高纯度分离的 His 标签蛋白。性能与其他供应商所提供的 Ni-NTA 磁珠相当或高于后者。
仅供科研使用。不可用于诊断程序。
规格
最大浓度Slurry: 12.5mg/mL, 1.25% solids
配基类型镍-NTA
蛋白质形式重组
数量10 mL
靶标His
容量（公制）10 mL
粒径1 μm
产品线HisPur
类型磁珠
Unit SizeEach
内容与储存
在 4°C 下储存

常见问题解答 (FAQ)

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between HisPur Nickel Resin and HisPur Cobalt Resin?

Nickel has a higher affinity for histidines than does cobalt. It binds multiple histidines more tightly than cobalt, and requires more stringent conditions than is necessary for cobalt. The lower affinity of cobalt for multiple histidines typically results in less nonspecific binding of histidine-rich proteins that lack a his-tag compared to nickel resins.

HisPur Ni-NTA resin has a high capacity of up to 60 mg of 6xHis-tagged protein per milliliter. It is a versatile resin that can work under both native and denaturing conditions, and can also be used with a variety of lysis reagents and buffer additives.

HisPur Cobalt resin utilizes proprietary tetradentate chelating resin charged with cobalt. This system recovers highly purified protein with lower imidazole concentrations and has low metal leeching properties.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用和文献 (3)

引用和文献
Abstract
Structure and bioactivity of an insecticidal trans-defensin from assassin bug venom.
Authors:Walker AA,Chin YK,Guo S,Jin J,Wilbrink E,Goudarzi MH,Wirth H,Gordon E,Weirauch C,King GF
Journal:Structure (London, England : 1993)
PubMed ID:38889720
Disulfide-rich peptides such as defensins play diverse roles in immunity and ion channel modulation, as well as constituting the bioactive components of many animal venoms. We investigated the structure and bioactivity of U-RDTX-Pp19, a peptide previously discovered in venom of the assassin bug Pristhesancus plagipennis. Recombinant Pp19 (rPp19) was found ... More
Human serum-derived α-synuclein auto-antibodies mediate NMDA receptor-dependent degeneration of CNS neurons.
Authors:Garg P,Würtz F,Hobbie F,Buttgereit K,Aich A,Leite K,Rehling P,Kügler S,Bähr M
Journal:Journal of neuroinflammation
PubMed ID:38419079
BACKGROUND: Presence of autoantibodies against α-synuclein (α-syn AAb) in serum of the general population has been widely reported. That such peripheral factors may be involved in central nervous system pathophysiology was demonstrated by detection of immunoglobulins (IgGs) in cerebrospinal fluid and brain of Parkinson's disease (PD) patients. Thus, blood-borne IgGs ... More
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells.
Authors:Stepanov AV,Xie J,Zhu Q,Shen Z,Su W,Kuai L,Soll R,Rader C,Shaver G,Douthit L,Zhang D,Kalinin R,Fu X,Zhao Y,Qin T,Baran PS,Gabibov AG,Bushnell D,Neri D,Kornberg RD,Lerner RA
Journal:Nature biomedical engineering
PubMed ID:37798444
On-target off-tumour toxicity limits the anticancer applicability of chimaeric antigen receptor (CAR) T cells. Here we show that the tumour-targeting specificity and activity of T cells with a CAR consisting of an antibody with a lysine residue that catalytically forms a reversible covalent bond with a 1,3-diketone hapten can be ... More