GeneChip™ Rat Genome 230 2.0 Array
GeneChip™ Rat Genome 230 2.0 Array
Applied Biosystems™

GeneChip™ Rat Genome 230 2.0 Array

GeneChip™ Rat Genome 230 2.0 Array 是毒理学、神经生物学及其他利用大鼠作为模式生物的应用的强大工具。•单一阵列可完全覆盖转录的大鼠基因组• 包含超过了解更多信息
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货号阵列数量
90050730 阵列
9005066 阵列
货号 900507
价格(CNY)
-
阵列数量:
30 阵列
GeneChip™ Rat Genome 230 2.0 Array 是毒理学、神经生物学及其他利用大鼠作为模式生物的应用的强大工具。

•单一阵列可完全覆盖转录的大鼠基因组
• 包含超过 31,000 个探针集,可分析超过 28,000 个确证大鼠基因的超过 30,000 个转录本和变体
• 通过 NetAffx™ 分析中心提供复杂的生物信息学工具,可以快速获得有生物学意义的结果
• 使用公开大鼠基因组草案数据和位居前列的公开大鼠数据库来完善序列,并提供更高质量的数据输出

阵列概况
GeneChip Rat Genome 230 2.0 Array 中包含了 GeneChip 大鼠表达集 230 上的所有探针集。在 GeneChip Rat Genome 230 2.0 Array 设计中使用的序列选自 GenBank™、dbEST 和 RefSeq。序列簇创建自 UniGene 数据库(Build 99,2002 年 6 月),然后通过分析和与公开可用的大鼠基因组草案数据(来自 Baylor College of Medicine Human Genome Sequencing Center [2002 年 6 月])进行比较来改进。

GeneChip 大鼠基因组 230 2.0 阵列包括 RefSeq 数据库代表性序列和与序列相关的探针集,以及之前显示于 GeneChip 大鼠基因组 U34 集并经过完善的 EST 簇。

在阵列上原位合成了与每个相应序列互补的寡核苷酸探针。使用十一对寡核苷酸探针来测量 GeneChip Rat Genome 230 2.0 Array 上的每个序列的转录水平。
仅供科研使用。不可用于诊断程序。
规格
产品线GeneChip™
数量30 arrays
类型大鼠基因组 230 2.0 阵列
数组转录组谱分析
产品规格阵列检测盒
阵列数量30 阵列
种属大鼠
Unit SizeEach

常见问题解答 (FAQ)

What reagent kit should I use with my array?

Please refer to the Microarray Reagent Guide for Arrays and Expression Kits to match the correct reagents your array.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How are transcript IDs assigned for WT arrays if a gene has multiple transcript IDs?

The first assigned mRNA/gene that is listed in the annotation file for a given probe set or transcript cluster is considered the "best" assignment based on data source quality ranking and assignment scoring system. To get a single best mRNA/gene assignment you can simply take the first one in the list in the annotation file.

Sometimes, there might be multiple best hits between transcript clusters (or probe set) and a mRNA/gene, with identical score and data source quality. In that case, the transcript assignments are ranked based on their descriptions (e.g., title from the GenBank record).

For transcript clusters that detect genes that are alternatively spliced, the different spliced forms will be ranked by length with the longest ones listed first.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Does the GeneChip miRNA 3.1 Array Strip require a different hyb/wash/stain kit than the GeneChip 3' IVT arrays and GeneChip ST array strips?

The hyb/wash/stain kit for the GeneChip miRNA 3.1 Array Strips is different than that for the 3' IVT arrays and GeneChip ST array strips. Additional wash A steps have been added to ensure adequate washing after the array strips have been stained.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What do the suffixes mean at the end of each probeset in GeneChip 3' IVT arrays?

The probe set names never change, but they can give you an idea of what was known about the sequence at the time of design.
_at: All probes hit one known transcript.
_a: All probes in the set hit alternate transcripts from the same gene.
_s: All probes in the set hit transcripts from different genes.
_x: Some probes hit transcripts from different genes.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I store my GeneChip 3' IVT Array after hybridization?

Yes, but we recommend storing your GeneChip 3' IVT Array after hybridization and prior to the fluidics step, for no longer than 3 hours at 4 degrees C. Please remove the hybridization cocktail and fill with wash A before storing.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.