B-PER™ 细菌蛋白提取试剂

在富营养培养基（高糖）中生长的细菌往往难以裂解。您可以尝试减少葡萄糖浓度。B-PER试剂能够裂解包括E. coli，Acetinobacter，Archaebacteria，S. aureus，H. pylori，昆虫杆状病毒，线虫和昆虫细胞在内的细菌和细胞。B-PER试剂可以裂解一些革兰氏阳性菌，但是裂解革兰氏阴性细菌的效率通常更高。如果对某一特定细菌菌株裂解效果不佳，则请在裂解前冷冻细菌。添加溶菌酶是最有效的提取手段，但是对于一些过表达蛋白则不需要加入溶菌酶。

B-PER II Reagent contains more detergent making it ideal for extracting proteins from small bacterial cultures with less than 20 mL in volume.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

B-PER Reagent utilizes a proprietary, mild nonionic detergent in a 20 mM Tris HCl, pH 7.5 buffer. No enzymatic components are present in the B-PER solution itself (B-PER Complete Bacterial Protein Extraction Reagent, Cat. No. 89821, 89822 does contain enzymes). Depending on your particular protein, you may need to add components such as salt, lysozyme, protease inhibitors, reducing agents and chelating agents. Other ready-to-use formats include B-PER II Bacterial Protein Extraction Reagent (2X), B-PER Reagent (in Phosphate Buffer), and B-PER with Enzymes Bacterial Protein Extraction Kit.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For 1 gram of cells add 4 mL of B-PER Reagent.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

While this is protein-dependent, many proteins have been successfully tested in downstream applications (reporter assays, immunoprecipitation and beta-Gal assays), including GST and 6xHis proteins. Otherwise, samples can be diluted or dialyzed to remove any interfering substances.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.