红色/绿色 BODIPY™ PC-A2(1-O-(6-BODIPY™ 558/568-氨基己基)-2-BODIPY™ FL C5-Sn-丙三基-3-磷酸胆碱)(比率式磷脂酶 A2 底物)
红色/绿色 BODIPY™ PC-A2(1-O-(6-BODIPY™ 558/568-氨基己基)-2-BODIPY™ FL C5-Sn-丙三基-3-磷酸胆碱)(比率式磷脂酶 A2 底物)
Invitrogen™

红色/绿色 BODIPY™ PC-A2(1-O-(6-BODIPY™ 558/568-氨基己基)-2-BODIPY™ FL C5-Sn-丙三基-3-磷酸胆碱)(比率式磷脂酶 A2 底物)

Molecular Probes™ 磷脂酶底物是使用染料标记的磷脂,用于监测纯化的酶制备物、细胞裂解物和活细胞中的磷脂酶 A (PLA) 活性。这些底物有两种结构变体,分别特异性用于 PLA1了解更多信息
Have Questions?
货号数量
A10072100 µg
货号 A10072
价格(CNY)
4,888.00
Each
添加至购物车
数量:
100 µg
价格(CNY)
4,888.00
Each
添加至购物车
Molecular Probes™ 磷脂酶底物是使用染料标记的磷脂,用于监测纯化的酶制备物、细胞裂解物和活细胞中的磷脂酶 A (PLA) 活性。这些底物有两种结构变体,分别特异性用于 PLA1 和 PLA2。这些底物生成的切割产物的荧光标记剂型也可用作标准品,用于评估酶反应中底物转化的百分比。

荧光磷脂酶 A 底物规格:

• 标记(激发/发射波长):红色/绿色 BODIPY™ PC-A2 (∼488/530,590 nm)(可用于双发射荧光比率检测)
• 特异性:PLA2
• 切割产物:D3834
• 冻干产品可溶于 DMSO 后使用
•荧光可通过微孔板检测或使用流式细胞分析检测

查找更多用于脂质代谢和脂肪酸类似物的探针
请参阅《Molecular Probes™ 手册》中的脂肪酸类似物和磷脂—第 13.2 节以及用于脂质代谢和信号转导的探针—第 17.4 节,了解有关这些产品的更多信息。

供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性细胞通透性
适用于(设备)流式细胞仪、微孔板读数仪
产品线BODIPY™
数量100 µg
运输条件室温
底物磷脂酶 A2 底物(比率测量)
检测方法荧光
形式实心
底物属性脂基底物
目标酶磷脂酶
Unit SizeEach
内容与储存
在冰箱(-5 至 -30°C)中储存。

常见问题解答 (FAQ)

The FRET-based substrate Red/Green BODIPY PC-A2 shows no change in fluorescence upon enzymatic cleavage. I'm not seeing an increasing green signal with higher concentrations of phospholipase A2 and no change in red fluorescence. Why is this?

There are numerous factors that can interfere with FRET-based detection. Here are a few items to consider:

a) Is there any component in the reaction solution that can quench green fluorescence?
b) Is there any component in the reaction solution that autofluoresces in the green or red emission range?
c) Too high of a substrate concentration may result in diffusional FRET: Lower the final working concentration of the substrate.
d) Ensure adequate dispersal of the substrate during the reaction. BODIPY dyes are very hydrophobic, and aggregates of the green product, BODIPY FL, can form an excimer and emit in the red range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

You provide Phospholipase A2 from honey bee venom in the EnzChek Phospholipase A2 Assay Kit. Can the kit be used with phospholipase A2 from other species?

Yes, phospholipase A2 from other species may be used as long as the phospholipase A2 can hydrolyze the sn-2 ester linkage of phospholipids and the enzymatic reaction can be done within a pH of >3 and <9.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.