引用和文献 (3)

Gibco™

CELLstart™ 底物

货号	数量
A1014201	2 mL

货号 A1014201

价格（CNY)

5,695.00

价格（CNY)

5,695.00

CELLstart Substrate is a defined substrate that supports human embryonic stem cell (hESC) attachment and expansion of undifferentiated colonies in serum-free medium without the need for feeder cells. It can also be used with mesenchymal and neural stem cells and for the attachment of human feeder cells (e.g., foreskin fibroblasts).

Defined substrate for stem cell culture
cGMP manufactured to help ensure manufacturability and traceability

Gibco™ products are of high quality and are supplied with harmonized documentation such as certificates of analysis and certificates of origin.

CELLstart™ 是一种成分确定的底物，仅含有人源性组分（无异种成分）。

用于干细胞培养的成分确定的无异种成分底物
按照 cGMP 标准进行生产，有助于确保可生产性和可追溯性

CELLstart™ 是一种成分确定的底物，仅含有人源性组分（无异种成分）。CELLstart™ 支持无血清培养基中人胚胎干细胞 (hESC) 的附着和未分化克隆的扩增，无需使用饲养层细胞。CELLstart™ 也可用于间充质干细胞、神经干细胞和人饲养层细胞（如包皮成纤维细胞）的附着。

Gibco™ 产品具有高质量，并附有统一文件，如检验报告和原产地证书。

仅供科研使用。不可用于诊断程序。

细胞类型干细胞(神经元),干细胞(iPS - 诱导性多能性干细胞),干细胞(胚胎),干细胞(间充质)

分类无血清

内毒素水平≤50 EU/mL、≤ 50 EU/mL

产品规格管

酚红指示剂无酚红

产品线Gibco

纯度或质量等级hESC 合格

数量2 mL

有效期24 months from date of manufacture

运输条件室温

Unit SizeEach

内容与储存

在 2°C 至 8°C 下存储。

常见问题解答 (FAQ)

间充质干细胞（MSC）研究中一般使用何种生长因子？

用于MSC培养和自我更新的生长因子：LIF（PHC9484），bFGF（PHG0261），EGF（PHG0311），HGF（PHG0324），PDGF（PHG0043）和Wnt3。
用于MSC分化的生长因子或细胞因子：BMP-2（PHC7145）与TGF-β1（PHG9211）。
(Arthritis Res Ther 9:204 (2007)).

我如何在体外培养间充质干细胞（MSC）？

MSC可培养在含或不含血清的培养基条件下。标准的培养条件为含10% FBS的低葡糖DMEM。MSC也可培养于低减血清（2%）的Gibco MesenPRO RS培养基（货号12746-012），或无血清的Gibco StemPro MSC SFM XenoFree培养基（货号A10675）和Gibco StemPro MSC SFM（货号A10332）中。通常情况下，MSC在含氧量较低的条件下（2% O2）生长得较好（2% O2）。

你们如何鉴定人间充质干细胞（MSC）？

可通过流式细胞术来鉴定人体MSC，这些细胞通常具有CD73+，CD90+，CD105+，CD11b-和CD45-的标志物属性（Blood 109:4245 (2007)）。人MSC表达Oct4，Sox2和Rex-1；这些mRNA均可通过RT-PCR技术进行鉴定（Arthritis Res Ther 9:204 (2007)）。

什么是间充质干细胞（MSC）？

间充质干细胞（MSC）是最初从骨髓或脂肪组织中分离出来的多专能细胞，它们表现出进一步分化为骨，软骨和脂肪细胞的能力。在常规细胞培养条件下，从骨髓中分离出的MSC为纺锤形，并拥有贴附于未包被塑料培养皿上的独特能力（Arthritis Res Ther 9:204 (2007)）。

我应如何鉴定人胚胎干（ES）细胞？

人体ES细胞一般可通过以下方法进行鉴定：经典型的形态（它们以致密的细胞团形式生长生长为紧密聚集的小型细胞团，细胞小并具有高核质比）；表面标志物的表达；干细胞特异性基因表达的RT-PCR检测（如Oct3/4，Sox2和Nanog）；碱性磷酸酶染色和端粒酶活性检测。最为常用的人ES细胞特异性表面标志物包括发育阶段特异性的胚胎抗原SSEA-3和SSEA-4。其它的ES细胞特异性的其它表面抗原还包括TRA-1-60和TRA-1-81。（Science 282:1145 (1998)）。

View all CELLstart™ 底物 FAQs

引用和文献 (3)

引用和文献

Abstract

CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice.

Authors:Haus DL, Nguyen HX, Gold EM, Kamei N, Perez H, Moore HD, Anderson AJ, Cummings BJ

Journal:

PubMed ID:25082219

'Common methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). Additionally, sufficient expansion of resulting hNSCs for scaling out or up ... More

Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.

Authors:Oikonomopoulos A, van Deen WK, Manansala AR, Lacey PN, Tomakili TA, Ziman A, Hommes DW,

Journal:

PubMed ID:26564250

'Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in ... More

Xeno-free defined conditions for culture of human embryonic stem cells, neural stem cells and dopaminergic neurons derived from them.

Authors:Swistowski A, Peng J, Han Y, Swistowska AM, Rao MS, Zeng X,

Journal:PLoS One

PubMed ID:19597550

BACKGROUND: Human embryonic stem cells (hESCs) may provide an invaluable resource for regenerative medicine. To move hESCs towards the clinic it is important that cells with therapeutic potential be reproducibly generated under completely defined conditions. METHODOLOGY/PRINCIPAL FINDINGS: Here we report a four-step scalable process that is readily transferable to a ... More