Alexa Fluor™ 568 酰肼，用于显微注射
Alexa Fluor™ 568 酰肼，用于显微注射
引用和文献 (21)
Invitrogen™

Alexa Fluor™ 568 酰肼，用于显微注射

Alexa Fluor™ 568 酰肼是一种细胞示踪剂，也是一种用于标记多糖或糖蛋白中醛或酮的反应性染料。该版本以即用型溶液的形式提供，溶解在 200 mM KCl 溶液中并经过除菌过滤。Alexa了解更多信息
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货号数量
A10441
又称 A-10441
125 μL
货号 A10441
又称 A-10441
价格（CNY)
5,462.00
Each
添加至购物车
数量:
125 μL
价格（CNY)
5,462.00
Each
添加至购物车
Alexa Fluor™ 568 酰肼是一种细胞示踪剂，也是一种用于标记多糖或糖蛋白中醛或酮的反应性染料。该版本以即用型溶液的形式提供，溶解在 200 mM KCl 溶液中并经过除菌过滤。

Alexa Fluor™ 568 是一种明亮的红色荧光染料。Alexa Fluor™ 568 染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性（pH 值 4 至 pH 值 10）。除反应性染料制剂外，我们还提供可与多种抗体、肽、蛋白、示踪剂和扩增底物偶联并且针对细胞标记和检测进行优化的 Alexa Fluor™ 568 染料（了解更多信息）。

关于该 AlexaFluor™ 酰肼的详细信息：

•荧光基团标记：Alexa Fluor™ 568 染料
•反应性基团：酰肼
•反应性：多糖或糖蛋白中的醛或酮
•偶联物的 Ex/Em：576/599 nm
• 消光系数：86,000 cm-1M-1
• 光谱相似染料：罗丹明红
• 分子量：730.74

细胞示踪和示踪应用
Alexa Fluor™ 酰肼和羟胺可用作低分子量、膜不可透过性、醛活化固定细胞示踪剂，具有比其他光谱相似荧光基团产生的细胞示踪剂更明亮的荧光和更高的光稳定性。它们可通过显微注射、从膜片移液管注入或通过我们的 Influx™ 胞饮细胞上样试剂所诱导的摄取进入细胞。其设计用于通过显微注射或膜片移液管注入细胞。了解更多有关细胞追踪和示踪的信息。

糖蛋白和多糖标记应用
Alexa Fluor™ 酰肼和羟胺是反应性分子，可用于向含有醛或酮的生物分子添加荧光标记。醛和酮可通过高碘酸盐介导的邻二醇氧化而引入多糖和糖蛋白中。半乳糖氧化酶也可用于将糖蛋白的末端半乳糖残基氧化为醛。

酰肼与羟胺
肼衍生物与酮和醛反应从而生成相对稳定的腙。羟胺衍生物（氨基氧基化合物）与醛和酮反应生成肟。肟的水解稳定性优于腙。使用硼氢化钠 (NaBH4) 可以还原腙和肟，以进一步提高键的稳定性。

了解有关蛋白和抗体标记的更多信息
我们可提供多种可供选择的 Molecular Probes ™ 抗体和蛋白标记试剂盒，以适应您的起始料和实验设置。参见我们的抗体标记试剂盒或使用我们的标记化学选择工具进行其他选择。欲了解有关我们标记试剂盒的更多信息，请参阅 Molecular Probes™ 手册中第 1.2 节—蛋白和核酸标记试剂盒。

我们还’可为您定制偶联物
如果您’无法在我们的在线目录中找到’想要的产品，我们还’可为您定制抗体或蛋白偶联物。我们的定制偶联服务是高效和保密的，我们保证我们的工作质量。我们经过 ISO 9001:2000 认证。

相关产品
DMSO（二甲亚砜）(D12345)
0.5-1 mg 用抗体偶联物纯化试剂盒 (A33086)
20-50 µg 用抗体偶联物纯化试剂盒 (A33087)
50-100 µg 用抗体偶联物纯化试剂盒 (A33088)
仅供科研使用。不可用于诊断程序。
规格
化学反应性羧酸、酮、醛
发射599 nm
激发576 nm
标签或染料Alexa Fluor™ 568
产品类型酰肼
数量125 μL
反应一部分胺、酰肼
运输条件室温
标签类型Alexa Fluor 染料
产品线Alexa Fluor
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

What dye can I use that is non-reactive and can show an injection site?

The non-reactive Alexa Fluor and Alexa Fluor hydrazide derivatives may be used for injection site visualization. Other options include the fluorescent polystyrene microspheres, FluoSpheres, and dye-conjugated dextrans. The hydrazide derivatives and 'fixable' dextrans are retained by cross-linking using an aldehyde-based fixative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (21)

引用和文献
Abstract
Intracellular astrocyte calcium waves in situ increase the frequency of spontaneous AMPA receptor currents in CA1 pyramidal neurons.
Authors:Fiacco TA, McCarthy KD
Journal:J Neurosci
PubMed ID:14736858
'Spontaneous neurotransmitter release and activation of group I metabotropic glutamate receptors (mGluRs) each play a role in the plasticity of neuronal synapses. Astrocytes may contribute to short- and long-term synaptic changes by signaling to neurons via these processes. Spontaneous whole-cell AMPA receptor (AMPAR) currents were recorded in CA1 pyramidal cells ... More
Characterization of a synaptiform transmission between a neuron and a glial cell in the leech central nervous system.
Authors:Britz FC, Lohr C, Schmidt J, Deitmer JW
Journal:Glia
PubMed ID:11968059
'The cross-talk between neurons and glial cells is receiving increased attention because of its potential role in information processing in nervous systems. Stimulation of a single identifiable neuron, the neurosecretory Leydig interneuron in segmental ganglia of the leech Hirudo medicinalis, which modulates specific behaviors in the leech, evokes membrane hyperpolarization ... More
Robust coding of flow-field parameters by axo-axonal gap junctions between fly visual interneurons.
Authors:Cuntz H, Haag J, Forstner F, Segev I, Borst A
Journal:Proc Natl Acad Sci U S A
PubMed ID:17551009
'Complex flight maneuvers require a sophisticated system to exploit the optic flow resulting from moving images of the environment projected onto the retina. In the fly''s visual course control center, the lobula plate, 10 so-called vertical system (VS) cells are thought to match, with their complex receptive fields, the optic ... More
Long-term potentiation of exogenous glutamate responses at single dendritic spines.
Authors:Bagal AA, Kao JP, Tang CM, Thompson SM
Journal:Proc Natl Acad Sci U S A
PubMed ID:16186507
'Long-term increases in the strength of excitatory transmission at Schaffer collateral-CA1 cell synapses of the hippocampus require the insertion of new alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) into the synapse, but the kinetics of this process are not well established. Using microphotolysis of caged glutamate to activate receptors at single dendritic spines in ... More
Three-dimensional reconstruction of tubular structure of vacuolar membrane throughout mitosis in living tobacco cells.
Authors:Kutsuna N, Kumagai F, Sato MH, Hasezawa S
Journal:Plant Cell Physiol
PubMed ID:14581629
'Plant vacuoles are the largest of organelles, performing various functions in cellular metabolism, morphogenesis and cell division. Dynamic changes in vacuoles during mitosis were studied by monitoring tubular structure of vacuolar membrane (TVM) in living transgenic tobacco BY-2 cells stably expressing a GFP-AtVam3p fusion protein (BY-GV). Comprehensive images of the ... More
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