含 dNTP 的 SequalPrep™ 长片段 PCR 试剂盒
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Applied Biosystems™

含 dNTP 的 SequalPrep™ 长片段 PCR 试剂盒

含 dNTP 的 SequalPrep™ Long PCR 试剂盒具有极高的扩增成功率(与在多种微生物和平台上针对长达 20 kB了解更多信息
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货号数量
A104981,000 units
货号 A10498
价格(CNY)
-
数量:
1,000 units
含 dNTP 的 SequalPrep™ Long PCR 试剂盒具有极高的扩增成功率(与在多种微生物和平台上针对长达 20 kB 的未优化扩增子进行测试的任意长片段 PCR 试剂盒相比),还无需优化。成功的远程 PCR 与最小优化,高效扩增 PCR 的各种应用,包括在下一代测序 (NGS) 平台上进行医学再测序。
PCR 扩增是传统的方法,用于丰富目标区域内基因组的基因分型和重新测序。为了利用增强的 NGS 处理能力,有必要采用更高通量的富集方法。不幸的是,利用现有的商业解决方案进行长片段 PCR 的潜在收益被较低的扩增成功率所抵消,富集仅限于 ∼5 kB 区域。含 dNTP 的 SequalPrep™ Long PCR 试剂盒克服了这些问题并具有极高的扩增成功率(与在多种微生物和平台上针对长达 20 kB 的扩增子进行测试的任意商用试剂盒相比),还无需优化。

该试剂盒可用于任何 NGS 平台上游或其他基因分型应用。当与 SequalPrep™ 归一化板结合使用时,SequalPrep™(序列均衡样本制备)系统是第一个完整的 PCR 富集和扩增子平衡解决方案、该解决方案可提供一种可行且高效的富集方法,以满足 NGS 重新测序通量能力。此 NGS 样本制备系统可显著减少 PCR 扩增和归一化的复杂性,从而允许以96孔板的格式富集长达 1MB 的区域借助 SequalPrep™ 的易用性进行放大和平衡,以持续高度覆盖您感兴趣的区域。
仅供科研使用。不可用于诊断程序。
规格
外切核酸酶活性3' - 5',5' - 3'
保真度(相对于 Taq)4 X
热启动内置热启动
突出端混合性
聚合酶SequalPrep 长片段聚合酶
产品线SequalPrep
数量1,000 units
反应形式分离组分
运输条件干冰
尺寸(最终产品)20 kb 或更小
高 GC PCR 扩增效果
反应速度标准
Unit SizeEach
内容与储存
• 200 μL SequalPrep™ Long 聚合酶 (5 U/μL)
• 1.2 ml SequalPrep™ 10X 反应缓冲液
• 1.2 mL SequalPrep™ 10X 增强剂 A
• 1.2 mL SequalPrep™ 10X 增强剂 B
• 750 μL DMSO

在 -20°C 下储存。DMSO 可在 4°C 或室温下储存。

常见问题解答 (FAQ)

What is the fidelity of the SequalPrep Long Polymerase in SequalPrep Long PCR Kit with dNTPs?

The SequalPrep Long PCR Kit with dNTPs demonstrates fidelity 3-4 times greater than recombinant Taq polymerase, similar to Invitrogen’s Platinum Taq High Fidelity Enzyme (by rpsl assay).  The SequalPrep Long PCR Polymerase was compared to rTaq, Platinum Taq HiFi, & Roche's Expand Long Template PCR System.  Relative fidelity:
• rTaq = 1
• Platinum Taq HiFi = 3.3
• Roche = 3.6
• SequalPrep Long with Enhancer A = 3.6
• SequalPrep Long with Enhancer B = 4.4

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

What organisms have been tested with SequalPrep Long PCR Kit with dNTPs kit?

Human and mouse genomic DNA have both been tested with the kit.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

SequalPrep Long PCR Kit with dNTPs comes with several enhancers. What are they and when should I use them?

The two Enhancers were selected to help provide maximum success rates. We recommend setting up the initial reactions with 0.5X Enhancer A to provide maximum success rate.  For any unsuccessful amplicons we recommend testing the following conditions to optimize amplification:
• 1.0X Enhancer A
• 0.5X Enhancer B
• 1.0X Enhancer B

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

How many units of the SequalPrep Long Polymerase should I use in a typical reaction for SequalPrep Long PCR Kit with dNTPs?

We recommend using 1.8 Units of SequalPrep Long Polymerase in a reaction.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

What amount of input material do you recommend for SequalPrep Long PCR Kit with dNTPs?

We recommend 5-200 ng of human genomic DNA and 20-80 ng of mouse embryonic stem cells DNA. 50 ng of DNA template at a concentration of 50 ng/µl should be sufficient for most applications, although input amounts as low as 10 ng can be possible depending on the sample type, due to the high yields generated by the kit.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.