CHO DG44 细胞（cGMP 保存）与培养基试剂盒
CHO DG44 细胞（cGMP 保存）与培养基试剂盒
Gibco™

CHO DG44 细胞（cGMP 保存）与培养基试剂盒

CHO DG44 细胞（cGMP 库存）与培养基试剂盒经优化用于二氢叶酸还原酶缺陷型 (DHFR-) 中国仓鼠卵巢 (CHO) 细胞的生长以及悬浮培养条件下的重组蛋白表达了解更多信息
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货号数量
A11000011 kit
货号 A1100001
价格（CNY)
237,717.00
Each
添加至购物车
数量:
1 kit
价格（CNY)
237,717.00
Each
添加至购物车
CHO DG44 细胞（cGMP 库存）与培养基试剂盒经优化用于二氢叶酸还原酶缺陷型 (DHFR-) 中国仓鼠卵巢 (CHO) 细胞的生长以及悬浮培养条件下的重组蛋白表达。该试剂盒提供：

GIBCO™ CD DG44 培养基—一种化学成分明确的无蛋白培养基，专门设计用于在悬浮培养中优化 DG44 细胞的生长和表达

CHO DG44 细胞（cGMP 库存）—已生产、建库和检测了亲代 CHO DG44 细胞，以符合 cGMP 质量标准。它们经预适应可用于 CD DG44 培养基，并经过选择以获得卓越的细胞生长和转染效率。

此外，CHO DG44 细胞（cGMP 库存）与培养基试剂盒包括 L-谷氨酰胺和 Pluronic™ F-68，用于在无蛋白、化学成分明确的环境中优化 DG44 细胞的生长和蛋白表达。
仅供科研使用。不可用于诊断程序。
规格
分类非动物源性、化学成分明确、无蛋白、无血清
推荐培养基CD DG44 培养基
池数量1 x 107
产品线Gibco
数量1 kit
细胞系CHO DG44
培养类型悬浮液细胞培养
种属仓鼠
Unit SizeEach
内容与储存
CHO DG44 细胞（cGMP 保存）与培养基试剂盒 (SKU A1100001) 包括：

•cd DG44 培养基 1000 mL - 12610-010，在黑暗环境中储存在 2-8°C
•CHO DG44 细胞（cGMP 库存）1 样品瓶（>1 x 10^7 个细胞）- A1097101（参见有限标签许可），在液氮中储存
•PLURONIC F-68 10% (100X) 100 mL - 24040-032，在室温下储存
•L-谷氨酰胺-200 mM (100X) 100 mL - 25030-081 或 25030-024（仅用于欧洲的 CE 认证版本），在 -20°C 下储存

常见问题解答 (FAQ)

Has the DG44 cell line been used for the large scale production of any FDA-approved biotherapeutic proteins?

70% of all therapeutics produced in mammalian cells are expressed in CHO cells. CHO cells are preferred because of human-like glycosylation and no propagation of human-like viruses. Most therapeutic IgGs are produced using the CHO platform and a number of FDA-approved biotherapeutics have been produced in DG44 cells.

Why are the defrosted CHO DG44 Cells (cGMP banked) transferred directly into pre-warmed media instead of being centrifuged for recovery when using the CHO DG44 Cells (cGMP banked) and Media Kit (Cat. No. A1100001)?

The defrosted contents of the vial of Gibco CHO DG44 Cells are transferred directly to 29 mL of pre-warmed media and then incubated because the cells are extremely fragile upon recovery from cryopreservation. Centrifuging the cells at this stage could cause significant damage, reducing their viability and recovery efficiency. To help ensure optimal recovery and viability, the cells are directly transferred to pre-warmed media and incubated under optimal conditions. This approach is also why we do not mention centrifuge resuspension in the subculture section. We have found that the shear forces during centrifugation are more harmful to the cells than retaining a small amount of old media or DMSO. The volume of media in the flask has been found to sufficiently dilute the DMSO such that it does not impact recovery or cell growth rather than using a centrifuge.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I learn more about commercial licensing of Freedom kits, ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

To inquire about a Commercial Production or Service License, please email: outlicensing@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the term of the license agreement for ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)?

Licensing rights are granted in perpetuity for the Commercial Production License. The term of the Commercial Service License is negotiated between the parties.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Is there a pull-through royalty on final products or an annual maintenance fee for using ExpiCHO-S Cells (cGMP banked), CHO-S Cells (cGMP banked), and CHO DG44 Cells (cGMP banked)??

Only a one-time license fee is required for a Commercial Production License, and it covers use during the clinical trial process and commercialization of an unlimited number of molecules.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

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