'Three-dimensional (3D) hydrogel structures are finding use in fundamental studies of self-assembly, rheology, and 3D cell culture. Most techniques for 3D hydrogel formation are ''single pot'', in which gels are not addressable after formation. For many applications, it would be useful to be able to form arrays of gels bearing ... More
Lumen Formation Is an Intrinsic Property of Isolated Human Pluripotent Stem Cells.
Authors:Taniguchi K, Shao Y, Townshend RF, Tsai YH, DeLong CJ, Lopez SA, Gayen S, Freddo AM, Chue DJ, Thomas DJ, Spence JR, Margolis B, Kalantry S, Fu J, O'Shea KS, Gumucio DL
Journal:
PubMed ID:26626176
'We demonstrate that dissociated human pluripotent stem cells (PSCs) are intrinsically programmed to form lumens. PSCs form two-cell cysts with a shared apical domain within 20 hr of plating; these cysts collapse to form monolayers after 5 days. Expression of pluripotency markers is maintained throughout this time. In two-cell cysts, ... More
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
Authors:Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman MJ, Kopetz S, Ellis LM, Meng QH, Li S
Journal:
PubMed ID:25516888
This study aimed to detect cell-surface vimentin (CSV) on the surface of epithelial-mesenchymal transitioned (EMT) circulating tumor cells (CTC) from blood of patients with epithelial cancers. ... More
Authors:TheinHan W, Liu J, Tang M, Chen W, Cheng L, Xu HH
Journal:
PubMed ID:24839581
Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferati on and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs ... More
Neural Differentiation of Spheroids Derived from Human Induced Pluripotent Stem Cells-Mesenchymal Stem Cells Coculture.
Authors:Song L, Tsai AC, Yuan X, Bejoy J, Sart S, Ma T, Li Y
Journal:Tissue Eng Part A
PubMed ID:29160172
'Organoids, the condensed three-dimensional (3D) tissues emerged at the early stage of organogenesis, are a promising approach to regenerate functional and vascularized organ mimics. While incorporation of heterotypic cell types, such as human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs)-derived neural progenitors aid neural organ development, ... More