仪器对照铽 TR-FRET 试剂盒
仪器对照铽 TR-FRET 试剂盒

仪器对照铽 TR-FRET 试剂盒

仪器对照铽 TR-FRET 试剂盒可用于基于铽 (Tb) 的 TR-FRET 检测的仪器设置快速评估。LanthaScreen™ 和 Premo™了解更多信息
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货号数量
A141381 mL kit
货号 A14138
价格(CNY)
2,859.00
Each
添加至购物车
数量:
1 mL kit
价格(CNY)
2,859.00
Each
添加至购物车
仪器对照铽 TR-FRET 试剂盒可用于基于铽 (Tb) 的 TR-FRET 检测的仪器设置快速评估。LanthaScreen™ 和 Premo™ 检测需要特定的仪器设置。正确的仪器设置对于实验的成功至关重要。该试剂盒含有 1 份高水平对照品和 1 份低水平对照品各一管,在运行 Tb TR-FRET 检测时也可用作阳性对照样品。

借助仪器对照铽 TR-FRET 试剂盒,您可以:

•设置仪器以快速读取 Tb TR-FRET 检测
•检查仪器 Tb TR-FRET 设置
•在每个检测板上读取高和低 Tb TR-FRET 信号对照品

确定您的仪器是否适当设置为读取 Tb TR-FRET 检测
适当设置您的仪器以读取 Tb TR-FRET 是成功运行 Tb TR-FRET 检测的第一步。该试剂盒允许您快速确定您的仪器是否已正确设置为读取 Tb TR-FRET 检测。只需将少量样品加入到单独的孔中,将微孔板放入仪器然后读取。如果高与低水平对照品信号的比值低于 2,仪器可能未正确设置。如果高与低水平对照品信号的比值介于 2 和 4 之间,您可以继续使用 Tb TR-FRET。

该试剂盒可作为 Tb TR-FRET 检测中的阳性对照品
在运行 Tb TR-FRET 检测时,可以在检测板上不同的孔中加入少量高水平和低水平对照品。按照说明将板放入仪器中并读取。2 至 4 的高与低水平对照品信号比值为您提供了仪器性能良好的信心。

仅供研究使用。不得用于任何动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色
适用于(设备)HTS Reader, Microplate Reader
产品线LanthaScreen
数量1 mL kit
建议的储存条件Store the kit at 4°C

Protect from light
运输条件Wet Ice
Unit SizeEach

常见问题解答 (FAQ)

I have no assay window in my TR-FRET assay. What is the reason?

The single most common reason that a TR-FRET assay fails is that an incorrect choice of emission filters was made. Unlike other fluorescent assays, the filters used in a TR-FRET assay must be exactly those that are recommended for your instrument as the emission filter choice can make or break the assay. The excitation filter has more of an impact on the assay window. Please refer to our instrument setup guides in our instrument compatibility portal (https://www.thermofisher.com/instrumentsetup). If your instrument is not listed there, please contact Drug Discovery Technical Support at drugdiscoverytech@thermofisher.com.

Note: Test your microplate reader's TR-FRET setup before you begin any work with your assay. Using reagents that you have already purchased for your assay, you can test the microplate reader's TR-FRET setup. Please refer to the Terbium (Tb) Assay (http://tools.thermofisher.com/content/sfs/manuals/LanthaScreen_Tb_Instrument_Control_Application_Note17Sep10.pdf) and Europium (Eu) Assay (http://tools.thermofisher.com/content/sfs/manuals/Eu_Instrument_Control_AppNote_12Nov10.pdf) Application Notes for plate reader setup.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.