Jump-In™ GripTite™ HEK 293 试剂盒
Jump-In™ GripTite™ HEK 293 试剂盒
Thermo Scientific™

Jump-In™ GripTite™ HEK 293 试剂盒

The Jump-In™ GripTite™ HEK293 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the了解更多信息
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货号数量
A141501 个试剂盒
货号 A14150
价格(CNY)
355,170.00
Each
添加至购物车
数量:
1 个试剂盒
价格(CNY)
355,170.00
Each
添加至购物车
The Jump-In™ GripTite™ HEK293 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ GripTite™ HEK293 cell line, to create an isogenic stable cell line with less effort and in less time than traditional cell engineering methods.

The high retargeting efficiency, made possible by the R4 sites in the GripTite™ HEK293 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ GripTite™ HEK293 Kit Lets You:

• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs
• Generate multiple cell lines in parallel using the simplified work flow
• Easily access the technology without complicated licenses or restrictions to interpret

Save Time with Rapid and Efficient Generation of Engineered Cell Lines
With the Jump-In™ GripTite™ HEK293 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined protocol makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

Expand Your Experimental Capabilities
The Jump-In™GripTite™ HEK293 Kit is the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to 'engineer' cell lines. The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway™ destination vectors accept large inserts.

The kit includes:
• pJTI™ R4 Dest CMV pA vector (100 μg)
• pJTI™ R4 Int vector (100 μg)
• Jump-In™GripTite™ HEK293 Cells (2 vials @ 1 ml each)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
仅供科研使用。不可用于诊断程序。
规格
细胞系Jump-In™ GripTite™ Hek293
输送类型转染
表达系统哺乳动物
适用于(应用)蛋白表达、测定试剂盒开发
关键功能稳定的细胞系开发、靶向整合
产品线Jump-In
产品类型HEK293 试剂盒
数量1 个试剂盒
选择试剂(真核生物)Geneticin™ (G-418),潮霉素
系统类型Jump-In™
产品规格试剂盒
促进剂CMV
Unit SizeEach
内容与储存
该试剂盒包括:
Jump-In™ GripTite™ HEK293 细胞
pJTI™ R4 Int(整合酶载体)
pJTI™ R4 DEST CMV pA(目的载体)

接收后,立即将细胞储存在液氮中,并将载体储存在 -20°C 下。储存在 -80°C 下的细胞可能会迅速丧失活力。

常见问题解答 (FAQ)

我试图建立一株Jump-In平台细胞系。你们是否推荐对整合位点进行定位?并筛选数据库以挑选一个在“良好”热点发生整合的克隆?

我们推荐您在用于建立平台细胞系的质粒中加入一个表达标志物/报告基因,之后通过该标志物的表达情况来筛选平台细胞系,以鉴定出一个高表达位点。否则,这一过程会非常繁琐,重定位后需要筛选大量的克隆。

pJTI PhiC31 Int载体是否包含了一个核定位信号(nuclear localization signal,NLS)?添加一个NLS是否能够增加拟attP位点的特异性整合效率?

pJTI Phic31 Int载体不含NLS信号。加入NLS信号可能会增加假attP位点的特异性整合效率,但尚未获得支持数据。有一篇文献描述了在PhiC31整合酶载体上NLS信号的应用,但作者未检测假attP位点的整合情况。

在Jump-In系统中,我需要使用多少DNA或何种对照品才能获得一个整合事件?

获得单拷贝所需的DNA用量可能要通过不含整合酶的对照试验来确定。在不含整合酶的条件下,生成低于5个克隆时的DNA用量可用于后续含整合酶的实验。一般来说,整合酶表达质粒占转染所需DNA量的大部分。

我应如何在Jump-In Fast系统和Jump-In TI系统间进行挑选?

如果您需要稳定的哺乳动物表达效果,并希望快速获得良好表达的克隆时,我们推荐您使用Jump-In Fast系统。您可通过PhiC31 假(pseudo)-att P位点上的一次或多次整合来获得良好表达的细胞克隆。用户有必要使用Southern杂交来确定整合的数目。如果您需要完成同基因的表达操作,则可选择Jump-In TI系统,这一系统可以确保所有的克隆基因都处于同一整合位点和背景水平,而不会出现染色体位置效应。

Jump-In与Flp-In系统之间的区别是什么?

Jump-In系统是由PhiC31-整合酶所介导的一个不可逆转的哺乳动物稳定定向表达系统。这一系统是由Jump-In Fast系统和Jump-In TI(定向整合)系统所组成,前者包含了单一的整合步骤,后者则需要两个整合步骤,两者都是定向且不可逆的。相比而言,Flp-In系统是一款可逆和稳定的哺乳动物靶向表达系统。第一步整合是随机的(pFRT/lacZeo的整合),而第二步整合(Flp-In表达载体的整合)是定向但可逆的。