Amplex™ Red 试剂
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Amplex™ Red 试剂
引用和文献 (224)
Invitrogen™

Amplex™ Red 试剂

Amplex Red 试剂，是高度灵敏和稳定的 H2O2 探针，是过氧化物酶最好的荧光底物。 存在辣根过氧化物酶 (HRP) 时，Amplex Red了解更多信息
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货号数量染料类型
A2217710 x 10 mgAmplex Red Reagent
A122225 mgAmplex Red Reagent
A360065 x 1 mgAmplex UltraRed Reagent
货号 A22177
价格（CNY)
43,028.00
Each
添加至购物车
数量:
10 x 10 mg
染料类型:
Amplex Red Reagent
价格（CNY)
43,028.00
Each
添加至购物车
Amplex Red 试剂，是高度灵敏和稳定的 H2O2 探针，是过氧化物酶最好的荧光底物。 存在辣根过氧化物酶 (HRP) 时，Amplex Red 试剂与 H2O2 以 1:1 的化学定量比反应，形成高荧光的试卤灵 (resorufin)。 因为很多不同的酶反应都会生成 H2O2，研究人员可以使用 Amplex Red 试剂检测各种酶的活性（Amplex Red 分析试剂盒）。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型Amplex Red Reagent
形式Solid
数量10 x 10 mg
运输条件Room Temperature
底物HRP (Horseradish Peroxidase) Substrate
底物类型Chemical substrate
经证实的应用Cell Analysis
适用于（应用）Hydrogen Peroxidase Assay
适用于（设备）Fluorometer, Microplate Reader
产品线Amplex
产品类型Red Hydrogen Peroxide
Unit SizeEach
内容与储存
在冰箱（-5 至 -30°C）中储存。

常见问题解答 (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have ordered and tested your Amplex Red Reagent (Cat. Nos. A12222, A22177), but it is not reacting correctly. What could be the cause?

Either the horseradish peroxidase or other enzymes in the assay may not be active, the H2O2 may have degraded, or the sample could have some component that is consuming H2O2 or inactivating the enzymes required for the assay. We recommend that you check when the reagent was purchased and how it was stored prior to use; the reagent should not be used past the warranty period.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you recommend using Amplex Red Reagent (Cat. Nos. A12222, A22177) in live cells?

Amplex Red is cell impermeant, but also its conversion to resorufin requires direct contact with the reaction of peroxidase with H2O2 (in solution, at the surface, or within cells). Within cells, peroxidases and H2O2 may not be sufficiently localized or active to provide enough signal.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use Amplex Red Reagent (Cat. Nos. A12222, A22177) in the presence of catalases?

Unless you are specifically analyzing catalase activity, as with the Amplex Red Catalase Assay Kit (Cat. No. A22180), the assay should be devoid of catalases. Catalases compete with the horseradish peroxidase for H2O2; the conversion of Amplex Red to resorufin requires the reaction of horseradish peroxidase with H2O2.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store Amplex Red Reagent (Cat. Nos. A12222, A22177)?

We recommend storing Amplex Red Reagent under vacuum (anaerobic conditions) and dehydrated. Do not store Amplex Red Reagent in solution.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Amplex™ Red 试剂 FAQs

引用和文献 (224)

引用和文献
Abstract
Roles for the human ATP-dependent Lon protease in mitochondrial DNA maintenance.
Authors:Lu B,Yadav S,Shah PG,Liu T,Tian B,Pukszta S,Villaluna N,Kutejová E,Newlon CS,Santos JH,Suzuki CK
Journal:The Journal of biological chemistry
PubMed ID:17420247
Pressure overload-induced mild cardiac hypertrophy reduces left ventricular transmural differences in mitochondrial respiratory chain activity and increases oxidative stress.
Authors:Kindo M, Gerelli S, Bouitbir J, Charles AL, Zoll J, Hoang Minh T, Monassier L, Favret F, Piquard F, Geny B,
Journal:Front Physiol
PubMed ID:22934079
Objective: Increased mechanical stress and contractility characterizes normal left ventricular (LV) subendocardium (Endo) but whether Endo mitochondrial respiratory chain complex activities is reduced as compared to subepicardium (Epi) and whether pressure overload-induced LV hypertrophy (LVH) might modulate transmural gradients through increased reactive oxygen species (ROS) production is unknown. Methods: LVH ... More
Overexpression of nicastrin increases Abeta production.
Authors:Murphy MP, Das P, Nyborg AC, Rochette MJ, Dodson MW, Loosbrock NM, Souder TM, McLendon C, Merit SL, Piper SC, Jansen KR, Golde TE
Journal:FASEB J
PubMed ID:12692078
Gamma-secretase cleavage is the final proteolytic step that releases the amyloid beta-peptide (Abeta) from the amyloid beta-protein precursor (APP). Significant evidence indicates that the presenilins (PS) are catalytic components of a high molecular weight gamma-secretase complex. The glycoprotein nicastrin was recently identified as a functional unit of this complex based ... More
Association of excitatory amino acid transporters, especially EAAT2, with cholesterol-rich lipid raft microdomains: importance for excitatory amino acid transporter localization and function.
Authors:Butchbach ME, Tian G, Guo H, Lin CL
Journal:J Biol Chem
PubMed ID:15187084
In the present study, we investigated the role of membrane cholesterol in the function of glutamate transporters. Depletion of membrane cholesterol by methyl-beta-cyclodextrin resulted in reduced Na(+)-dependent glutamate uptake in primary cortical cultures. Glial glutamate transporter EAAT2-mediated uptake was more sensitive to this effect. Cell surface biotinylation and immunostaining experiments ... More
The characterization of mitochondrial permeability transition in clonal pancreatic beta-cells. Multiple modes and regulation.
Authors:Koshkin V, Bikopoulos G, Chan CB, Wheeler MB
Journal:J Biol Chem
PubMed ID:15231823
Mitochondrial permeability transition (MPT), which contributes substantially to the regulation of normal mitochondrial metabolism, also plays a crucial role in the initiation of cell death. It is known that MPT is regulated in a tissue-specific manner. The importance of MPT in the pancreatic beta-cell is heightened by the fact that ... More
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