Neurobasal™-A 培养基,不含 D-葡萄糖,不含丙酮酸钠
Neurobasal™-A 培养基,不含 D-葡萄糖,不含丙酮酸钠
Gibco™

Neurobasal™-A 培养基,不含 D-葡萄糖,不含丙酮酸钠

在 D-葡萄糖和丙酮酸钠存在可能引起干扰的应用中,不含 D-葡萄糖和丙酮酸钠的 Neurobasal-A 培养基用于支持产后 CNS 神经元的生长。对于不需要胰岛素、葡萄糖和丙酮酸钠的神经研究(如葡萄糖反应或胰岛素反应研究了解更多信息
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货号数量
A2477501500 mL
货号 A2477501
价格(CNY)
1,509.00
Each
添加至购物车
数量:
500 mL
价格(CNY)
1,509.00
Each
添加至购物车
在 D-葡萄糖和丙酮酸钠存在可能引起干扰的应用中,不含 D-葡萄糖和丙酮酸钠的 Neurobasal-A 培养基用于支持产后 CNS 神经元的生长。

对于不需要胰岛素、葡萄糖和丙酮酸钠的神经研究(如葡萄糖反应或胰岛素反应研究),我们建议使用不含胰岛素的 Gibco B-27 补充剂作为该培养基的补充剂。

Neurobasal 培养基适用于大多数神经元细胞应用。对于其他应用,我们提供了替代配方的 Neurobasal 培养基B-27 添加剂

有关使用 Neurobasal 培养基的成熟实验方案,请查看本公司的 Gibco 神经生物学实验方案手册

如需查看使用 Gibco 神经生物学产品的出版物,请访问本公司的 Gibco CellCite

仅供科研使用。不可用于诊断程序。
规格
细胞类型神经细胞
产品线Neurobasal™
产品类型Neurobasal 培养基
数量500 mL
有效期自生产之日起 12 个月
运输条件环境
经过测试内毒素、性能、无菌性、渗透压、pH 值
分类无血清
培养类型神经元细胞培养
形式液体
加有添加剂酚红
不加添加剂无葡萄糖, 不含丙酮酸钠
Unit SizeEach
内容与储存
请避光冷藏 (2–8°C) 储存。

常见问题解答 (FAQ)

Can you provide references for growing adult neurons?

Instructions for growing adult neurons can be found in the Neurobasal-A Medium product insert. You can also refer to the following publication for growing adult neurons:
• Isolation and Culture of Adult Rat Hippocampal Neurons by Brewer, G.J., J. Neuroscience Methods 71 (1997), 145-158.

Should I use Neurobasal-A Medium with B27 and b-FGF for postnatal neurons?

We do not know at what age postnatal neurons require FGF and a higher osmolality medium, or how this could vary based on the species from which the neural tissue is isolated. Without this knowledge, we generally recommend switching to the Neurobasal-A Medium for isolates from animals above 1 week of age. You will need to determine if the Neurobasal-A Medium is optimal for neurons you have from one day old rats.

Further, the addition of bFGF can increase the viability of neurons of any age. Therefore, caution needs to be made in impure neuronal cell culture as bFGF can increase the proliferation of non-neuronal cells. We recommend a pilot experiment to test the impure culture in the presence and absence of bFGF.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What should I add to Neurobasal-A Medium to grow adult neurons?

We recommend adding B27 and b-FGF to Neurobasal-A Medium to grow adult neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What did you add to Neurobasal-A Medium to increase osmolality?

4 g/L of NaCl has been added to the Neurobasal-A Medium formulation to increase osmolality.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.