Alexa Fluor™ 647 微量蛋白标记试剂盒

Now available:Upgraded Alexa Fluor Antibody Labeling kits with user-friendly, advanced purification columns that ensure rapid dye removal and high yield of labeled proteins in just 30 minutes, with minimal hands-on time.

引用和文献 (4)

Invitrogen™

Alexa Fluor™ 647 微量蛋白标记试剂盒

微量蛋白标记试剂盒提供了一种便利的方法，可将荧光标记连接至少量抗体或蛋白 (20–100 µg)。试剂盒具有四种 Alexa Fluor™ 颜色（或生物素），提供三次标记和分离反应所需的全部试剂。微量蛋白标记试剂盒的重要特点：•标记蛋白通常可在了解更多信息

货号	数量
A30009	1 个试剂盒

货号 A30009

价格（CNY)

10,588.00

添加至购物车

1 个试剂盒

价格（CNY)

10,588.00

添加至购物车

微量蛋白标记试剂盒提供了一种便利的方法，可将荧光标记连接至少量抗体或蛋白 (20–100 µg)。试剂盒具有四种 Alexa Fluor™ 颜色（或生物素），提供三次标记和分离反应所需的全部试剂。

微量蛋白标记试剂盒的重要特点：
•标记蛋白通常可在 2 小时内立即可用（手动操作时间 ∼30 分钟）
•针对分子量介于 12 和 150 kDa 之间的 20–100 µg 蛋白进行了优化
•使用便利的离心过滤柱进行纯化，得率介于 60% 和 90% 之间
•标记前必须去除样品中的稳定蛋白

稳定反应化学和卓越 Alexa Fluor™ 染料
在微量蛋白标记试剂盒中，反应性染料含有琥珀酰亚胺 (NHS) 酯部分，该部分与蛋白的伯胺反应形成稳定的染料-蛋白偶联物。与传统染料相比，Alexa Fluor™ 染料更明亮、光稳定性更好，且在 pH 值 4 至 10 之间的 pH 值耐受性更强。通常，当使用 Alexa Fluor™ 染料时，可达到更高程度的标记，而无需分子内淬灭。有关详细信息，请参见跨越可见光谱和红外光谱的 Alexa Fluor™ 染料—第 1.3 节。

了解更多关于蛋白和抗体标记的信息
我们可提供多种可供选择的 Molecular Probes™ 抗体和蛋白标记试剂盒，以适应您的起始材料和实验设置。参见 A 至 Z 的抗体标记或使用我们的标记化学选择工具进行其他选择。要了解更多关于不同试剂盒的信息，请阅读 Molecular Probes™ 手册中标记蛋白和核酸的试剂盒—第 1.2 节。

我们将为您定制抗体偶联物
如果您在我们的储存列表中找不到您想要的产品，我们将为您制备定制抗体偶联物。我们的定制偶联服务高效且保密, 而且我们绝对保证质量。我们获得了 ISO 9001:2000 认证。

仅供研究使用。不适用于动物或人类的治疗或诊断。

仅供科研使用。不可用于诊断程序。

颜色远红外

检测方法荧光

激发/发射650/665

标签类型Alexa Fluor 染料

标记方法基于偶联

标记规模20 至 100 μg

产品线Alexa Fluor

产品类型蛋白标记试剂盒

数量1 个试剂盒

反应一部分琥珀酰亚胺 (NHS) 酯

运输条件室温

标记目标蛋白

标签或染料Alexa Fluor 647

Unit SizeEach

内容与储存

在冷藏冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

我应该使用多大浓度的抗体进行偶联？

抗体的浓度应该在1.0-3.0 mg/mL。抗体必须不含防腐剂（叠氮化物）、含胺的缓冲液以及BSA之类的载体蛋白。

何为标记度(DOL)?

标记度（DOL）指的是每个抗体所带的荧光团数量。对于体内标记实验，DOL被限制在一个狭窄范围内，因为它对生物分布和探针清除具有显著影响。我们已经确定远红外Alexa Fluor 染料用于活体成像的最佳光学DOL范围是1.5至3分子每个抗体。

Can I use 50 μg of protein with Fluorescent Protein Labeling Kits?

No. We recommend using 1 mg of protein with Fluorescent Protein Labeling Kits. For smaller protein sample sizes, we recommend using Microscale Protein Labeling kits which are optimized for 20-100 µg of protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What formulation of antibody should I use for conjugation for small animal in vivo imaging?

To allow for good reaction kinetics, antibodies should be in PBS buffer at a concentration of 0.5-3.0 mg/ml. The antibody must be free of preservatives (azide etc.), amine containing buffers and carrier proteins such as BSA.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is degree of labeling (DOL)?

Degree of labeling (DOL) describes the number of fluorophores per antibody. For in vivo labeling experiments, the DOL is restricted to a narrow range because it has significant consequences for the biodistribution and clearance of the probe. For example, for in vivo imaging, we have determined that the DOL range for the far-red Alexa Fluor dyes is 1.5 to 3 molecules per antibody for optimal optical in vivo imaging.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (4)

引用和文献

Abstract

Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.

Authors:Gupta R,Kus B,Fladd C,Wasmuth J,Tonikian R,Sidhu S,Krogan NJ,Parkinson J,Rotin D

Journal:Molecular systems biology

PubMed ID:17551511

Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protein microarray platform that can be utilized for the ... More

Mouse marginal zone B cells harbor specificities similar to human broadly neutralizing HIV antibodies.

Authors:Pujanauski LM, Janoff EN, McCarter MD, Pelanda R, Torres RM,

Journal:Proc Natl Acad Sci U S A

PubMed ID:23288906

'A series of potent, broadly neutralizing HIV antibodies have been isolated from B cells of HIV-infected individuals. VRC01 represents a subset of these antibodies that mediate neutralization with a restricted set of IGHV genes. The memory B cells expressing these antibodies were isolated years after infection; thus, the B-cell subpopulation ... More

Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.

Authors:Roether J, Bertels S, Oelschlaeger C, Bastmeyer M, Willenbacher N

Journal:PLoS One

PubMed ID:30566463

'As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required. We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells. Structure and elasticity of macroporous, three-dimensional cryogel ... More

Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing.

Authors:Tabdanov ED, Puram V, Zhovmer A, Provenzano PP

Journal:Cell Rep

PubMed ID:30304674

Cancer cell migration through and away from tumors is driven in part by migration along aligned extracellular matrix, a process known as contact guidance (CG). To concurrently study the influence of architectural and mechanical regulators of CG sensing, we developed a set of CG platforms. Using flat and nanotextured substrates ... More