Vitronectin (VTN-N) Recombinant Human Protein, Truncated
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IMPORTANT UPDATE: Vitronectin (VTN-N) Recombinant Human Protein, Truncated, now available under new catalog numbers, A400457 and A400458. Catalog numbers A14700 and A31804 will be discontinued.
Vitronectin (VTN-N) Recombinant Human Protein, Truncated
Gibco™

Vitronectin (VTN-N) Recombinant Human Protein, Truncated

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs).
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货号数量
A3180410 mL = 5 mg
A147001 mL = 0.5 mg
货号 A31804
价格(CNY)
8,749.90
飞享价
Ends: 31-Dec-2025
10,438.00
共减 1,688.10 (16%)
Each
添加至购物车
数量:
10 mL = 5 mg
价格(CNY)
8,749.90
飞享价
Ends: 31-Dec-2025
10,438.00
共减 1,688.10 (16%)
Each
添加至购物车

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8 Medium (Cat. No. A1517001), VTN-N has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines.

  • Proven to maintain pluripotency and normal growth characteristics in multiple PSC lines when used with Essential 8 Medium
  • Supports PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages
  • Supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8 Medium
  • Optimized—designed in the laboratory of James Thomson for use with Essential 8 Medium
  • Defined—for reduced variability in your PSC cultures
  • Supports human pluripotent stem cell attachment and survival better than wild type vitronectin
  • Optimal environment for feeder-free pluripotent stem cell culture
  • Defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs)
For Research Use Only. Not for use in diagnostic procedures.
规格
最大浓度500 μg/mL
表达系统E. coli
蛋白质形式Recombinant
蛋白质子类型Vitronectin
纯度或质量等级Cell Culture Grade, Endotoxin-Screened
数量10 mL = 5 mg
研究类别Stem Cell Research
运输条件Dry Ice
容积(公制)10 mL
适用于(应用)Stem Cell Culture
形式Liquid
重组Recombinant
种属Human
Unit SizeEach
内容与储存
Store at -80°C and protect from light.

常见问题解答 (FAQ)

之前冻存于其他培养条件的多能干细胞(PSCs)能复苏并后续培养于Gibco Essential 8培养基+玻连蛋白(VTN-N)的组合条件中么?

可以。在mTeSR培养基+BD Matrigel基底膜基质条件下培养并冻存的PSCs细胞可复苏至Gibco Essential 8培养基中并种植于VTN-N基质上。某些细胞系复苏至冻存前的生长培养基及基质中可能会效果更好。在随后的下一次传代过程中,就可使用EDTA将这些细胞传代至Gibco Essential 8培养基+VTN-N的组合中了。

在使用Gibco Essential 8培养基和玻连蛋白(VTN-N)的组合开展细胞培养时,我能否使用分散酶和胶原酶等酶类进行传代?

分散酶与胶原酶一类的酶并不适用于Gibco Essential 8培养基和Gibco玻连蛋白(VTN-N)的组合。使用这些酶类传代会导致细胞活力和贴壁能力下降。

Gibco Essential 8培养基和Gibco玻连蛋白(VTN-N)的组合中推荐用怎样的方法进行细胞传代?

培养在Gibco Essential 8培养基与VTN-N组合中的细胞应使用EDTA进行传代。

在Gibco Essential 8培养基 和Gibco玻连蛋白(VTN-N)的体系中培养的细胞外观如何?

您预期可看到正常的多能干细胞(PSC)形态。PSC的预期生长形态是如图所示的典型形态为,包括边界清晰的紧密聚集克隆和高核质比。

之前培养于其他培养基和基质的多能干细胞(PSC)能换用Gibco Essential 8培养基和Gibco玻连蛋白进行培养么?

培养于其它无饲养层培养体系的细胞,例如mTeSR培养基搭配Matrigel基底膜基质,或StemPro hESC SFM搭配Geltrex基质,也能够成功培养于Gibco Essential 8培养基搭配VTN-N的体系中。此外,在饲养层细胞搭配KnockOut SR中培养的PSC经证明也能够培养于Gibco Essential 8培养基搭配VTN-N的组合中。不过在更换培养基体系之前,细胞必须手工传代或用EDTA进行传代到Gibco Essential 8培养基搭配VTN-N的体系中。