Ion SingleSeq™ 96 试剂盒
Product Image
Ion Torrent™

Ion SingleSeq™ 96 试剂盒

Ion SingleSeq 96 试剂盒设计用于在拷贝数事件的低通基因组测序检测前进行低细胞数量文库构建。该试剂盒中包含多种试剂,只需单个细胞或多个细胞来源的低至 6 pg 的 DNA,便可提取、扩增基因组了解更多信息
Have Questions?
货号数量
A3476396 reactions
货号 A34763
价格(CNY)
-
数量:
96 reactions
Ion SingleSeq 96 试剂盒设计用于在拷贝数事件的低通基因组测序检测前进行低细胞数量文库构建。该试剂盒中包含多种试剂,只需单个细胞或多个细胞来源的低至 6 pg 的 DNA,便可提取、扩增基因组 DNA 并为其添加条形码,用于从多达 96 个样品中制备文库。

Ion SingleSeq 96 试剂盒可与适用于 Ion S5 系统的 Ion ReproSeq PGS 试剂盒配合使用,用于检测染色体非整倍体和染色体臂拷贝数事件 (>48 Mb)。Ion ReproSeq PGS 试剂盒可与 Ion Chef 系统和 Ion S5 或 Ion S5 XL 测序系统配合使用,采用一次性试剂盒实现自动化模板制备、芯片加载和测序,加快并简化仪器设置,较大限度地减少手动操作时间。兼容的试剂盒包括:
• Ion ReproSeq PGS 试剂盒,含 Ion 520 芯片(24 份样品/运行)(货号 A34900)
• Ion ReproSeq PGS 试剂盒,含 Ion 530 芯片(96 份样品/运行)(货号 A34901)
仅供科研使用。不可用于诊断程序。
规格
适用于(设备)Ion GeneStudio S5 系统、Ion PGM™ 系统、Ion Chef™ 系统
片段文库
产品类型96 个试剂盒
数量96 reactions
测序类型基因组 & DNA 测序
运输条件可置于湿冰或干冰上运输
通量96 个样品
工作流程步骤文库生成
产品线ReproSeq
原始材料DNA
Unit SizeEach
内容与储存
储存在 –10 至 –30°C 下。

常见问题解答 (FAQ)

Does the Ion SingleSeq 96 Kit include reagents for template preparation, sequencing, and chips?

No, the Ion SingleSeq 96 Kit only includes reagents to prepare the library. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion SingleSeq 96 Kit?

The Ion SingleSeq 96 Kit includes reagents to extract, amplify, and barcode genomic DNA for preparation of libraries from up to 96 samples. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Where can I find the sequences for the IonCode barcodes?

The sequences for the barcodes can be found in the Barcodes section of your Torrent Browser. You can also download a .csv file containing the full list of sequences in that set.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Are the Ion ReproSeq PGS Kit subkits available separately?

Yes, the Ion SingleSeq Kit (Cat. No. A28955, 24 reactions/kit), or Ion SingleSeq 96 Kit (Cat. No. A34763, 96 reactions/kit) are available separately.

The Ion ReproSeq PGS Kits - Ion S5 System, are also available separately and include reagents to prepare templates and load 4 chips on the Ion Chef Instrument, and sequence the chips on the Ion S5 or Ion S5 XL Sequencer.
- Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run), Cat. No. A34900
- Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run), Cat. No. A34901

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.