CF6-Neo 小鼠胚胎成纤维细胞，经过辐照

Gibco™

CF6-Neo 小鼠胚胎成纤维细胞，经过辐照

Irradiated Gibco CF6-Neo Mouse Embryonic Fibroblasts (MEFs) are neomycin-resistant feeder cells that support healthy undifferentiated human and mouse embryonic stem了解更多信息

货号	数量
A34963	4 x 10⁶ cells

货号 A34963

价格（CNY)

1,175.65

Ends: 31-Dec-2025

1,402.00

共减 226.35 (16%)

添加至购物车

4 x 10⁶ cells

价格（CNY)

1,175.65

Ends: 31-Dec-2025

1,402.00

共减 226.35 (16%)

添加至购物车

Irradiated Gibco CF6-Neo Mouse Embryonic Fibroblasts (MEFs) are neomycin-resistant feeder cells that support healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6005G, these MEF cells are isolated from CF1 x C57BL/6 hybrid mice, mitotically arrested by irradiation and tested to help ensure performance and minimize the risk of contamination. For neomycin-resistant MEFs that are inactivated by mitomycin C treatment, see CF6-Neo Mouse Embryonic Fibroblasts, MitC-treated. You may also choose from our broad selection of other MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform research involving drug selection using neomycin-resistant cells

Help save time with ready-to-use MEFs
Irradiated CF6-Neo MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF6-Neo MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to help ensure robust and consistent performance in your research. These MEFs have successfully been used with ESC/iPSC lines from other species but should be tested for compatibility if used for such an application.

Perform research involving drug selection using neomycin-resistant cells
CF6-Neo MEFs express neoR from a pPGKneobPa transgene, making them highly resistant to neomycin and geneticin (G418). Each lot is tested for tolerance to various G418 concentrations, and the threshold range for survival is reported in the CoA to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing G418 selection.

仅供科研使用。不可用于诊断程序。

供体混合供体

池数量4 x 10⁶ 个细胞

产品类型饲养细胞

数量4 x 10⁶ cells

运输条件用于干冰运输

细胞类型饲养层细胞、成纤维细胞

形式Cryopreserved

Unit SizeEach

内容与储存

冻存小鼠胚胎成纤维细胞

储存于液氮中。

常见问题解答 (FAQ)

Is it better to use irradiated mouse embryonic fibroblasts (MEFs) or Mitomycin C-treated MEFs to grow embryonic stem cells?

Either method will work in arresting cell division. However, the irradiation process will ensure that cell division will cease regardless of cell aggregation. Cell clumping can potentially not inactivate all cells when using mitomycin C, as cells within clumps may not be exposed to the mitomycin C. Irradiated cells are preferred by those who have concerns about chemical treatment. Mitomycin C-treated cells are preferred by those who have concerns about DNA damage from irradiation.

What are the differences between CF1, CF6, and DR4 mouse embryonic fibroblasts?

CF1 mouse embryonic fibroblasts do not have drug resistance. CF6 mouse embryonic fibroblasts are resistant to Neomycin/geneticin (G418). DR 4 mouse embryonic fibroblasts are resistant to geneticin (G418), puromycin, hygromycin, and 6-thioguanine.

What is the recommended seeding density for mouse embryonic fibroblasts? Why is the seeding density critical?

We recommend seeding these cells at densities ranging from 2 x 10E4 to 5.5 x 10E4 cells/cm2. A good starting point is 3 x 10E4 cells/cm2. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the feeder cells are too dense, the feeder layer may detach from the plate, and the culture will be lost.

When should mouse embryonic fibroblasts be plated and how long can these cells be used?

These cells should be plated 24 hours prior to plating the ESCs or iPSCs and should be used for only 7-10 days.

Does the culture vessel need to be coated prior to seeding mouse embryonic fibroblasts?

Yes, the culture vessel needs to be coated with Attachment Factor protein (Cat. No. S006100) at 37 degrees C for 30 mins or at room temperature for 2 hours. The coated vessels can be used immediately or stored at room temperature for up to 24 hours.