PNGase F 聚糖切割试剂盒

PNGase F 聚糖切割试剂盒包括从糖蛋白中酶去除几乎所有 N-连接低聚糖所必需的所有组分。该试剂盒包括 PNGase F 酶，它可以在高甘露糖、混合型和复杂低聚糖的最内端 GlcNAc了解更多信息

货号	数量
A39245	1 kit

货号 A39245

价格（CNY)

3,600.94

Ends: 31-Dec-2025

4,279.00

共减 678.06 (16%)

添加至购物车

1 kit

价格（CNY)

3,600.94

Ends: 31-Dec-2025

4,279.00

共减 678.06 (16%)

添加至购物车

PNGase F 聚糖切割试剂盒包括从糖蛋白中酶去除几乎所有 N-连接低聚糖所必需的所有组分。该试剂盒包括 PNGase F 酶，它可以在高甘露糖、混合型和复杂低聚糖的最内端 GlcNAc 和天冬酰胺残基处切割 N-聚糖链。

PNGase F 聚糖切割试剂盒的特点包括：
•≥ 95% 纯度
•无糖苷内切酶 F1、F2 或 F3 的重组 PNGase F 酶
•酶切割 N-聚糖核心低聚糖完整且适用于下游分析
•适于在天然或变性条件下使用

PNGase F 聚糖切割试剂盒中包括的组分为：30,000 单位 PNGase F（500,000 单位/mL）和 1 mL PNGase F 10X 缓冲液。

仅供科研使用。不可用于诊断程序。

数量1 kit

运输条件湿冰

产品类型PNGase F 聚糖切割试剂盒

Unit SizeEach

内容与储存

•30,000 单位 PNGase F 重组酶（500,000 单位/mL），在 -20°C 下储存
•1 mL PNGase F 10X 缓冲液，在 -20°C 下储存

常见问题解答 (FAQ)

In the PNGase Glycan Cleavage Kit, can I use PNGase F under native or denaturing conditions?

Yes, PNGase F enzyme may be used in both native and denaturing conditions

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, how much PNGase F should I use to remove my carbohydrate under native or DTT denaturing conditions?

When the protein is not denatured with SDS, PNGase F has to work harder to reach the cleavage site of the carbohydrate (due to secondary and tertiary protein structures). In these instances, using more enzyme and/or extended incubation times may help. These conditions will need to be determined empirically for your specific protein.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, is PNGase F compatible with downstream analysis such as HPLC and mass spectrometry?

PNGase F enzyme is stored in 50% glycerol. Therefore, HPLC and/or mass spectrometry is not compatible due to the fact that glycerol is not tolerated in instruments used for these applications.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, what are the typical reaction conditions for PNGase F, Recombinant?

Typical reaction conditions are as follows:

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate.
1. Combine 1-20 µg of glycoprotein, 1 µL of 10X denaturing buffer (5% SDS, 400 mM DTT) and H2O (if necessary) to make a 10 µL total reaction volume.
2. Denature glycoprotein by heating reaction at 100 degrees C for 10 min.
3. Make a total reaction volume of 20 µL by adding 2 µL PNGaseF 10X Buffer, 1-5 µL PNGaseF enzyme, 2 µL 10% NP-40 and H2O.
4. Incubate reaction at 37 degrees C for 1 hr.
Note: Reactions may be scaled-up linearly to accommodate larger reaction volumes.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

In the PNGase Glycan Cleavage Kit, what happens to the asparagine residue after PNGase F removes the sugar?

As PNGase F is a glycoamidase, the asparagine gets converted into aspartic acid.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.