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Expi293™ 诱导性 GnTI - 表达系统试剂盒
The Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from了解更多信息
| 货号 | 数量 |
|---|---|
| A41276 | 1 个试剂盒 |
货号 A41276
价格（CNY)
-
数量:
1 个试剂盒
The Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible GnTI– cell line was established by the selective knockout of the GnTI gene and the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter, in parental Expi293F cells. This cell line is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.
Expi293 Inducible GnTI– Expression System Kit features include:
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible GnTI– cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers
The components included in the Expi293 Inducible GnTI– Expression System Kit are: one vial of frozen Expi293F Inducible GnTI– cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, one pcDNA5/TO Mammalian Expression Vector kit, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible GnTI– Expression System are also available for individual purchase.
Expi293 Inducible GnTI– Expression System Kit features include:
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible GnTI– cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers
The components included in the Expi293 Inducible GnTI– Expression System Kit are: one vial of frozen Expi293F Inducible GnTI– cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, one pcDNA5/TO Mammalian Expression Vector kit, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible GnTI– Expression System are also available for individual purchase.
仅供科研使用。不可用于诊断程序。
规格
细胞系Expi293F™
输送类型转染
适用于（应用）蛋白表达
关键功能蛋白质制备
产品线Expi293
产品类型表达系统试剂盒
数量1 个试剂盒
运输条件经批准可在室温下或者湿冰或干冰上运输
细胞类型Expi293F
促进剂CMV/TO
样品类型DNA
Unit SizeEach
内容与储存
• Expi293F 诱导 GnTI 细胞（1 x 107 个细胞/小瓶），储存于汽相液氮中
• 1 L Expi293 表达培养基，在 2–8°C 下储存（避光储存）
• ExpiFectamine 293 转染试剂盒，用于 1 L 培养，在 2–8°C 下储存
• PNGase F 聚糖切割试剂盒，在 -20°C 下储存
• 100 mL Opti-Plex 综合缓冲液，在 2–8°C 下储存（避光）
• 20 µg pcDNA5/TO 哺乳动物表达载体，在 2–8°C 下储存或在 -20°C 下长期储存。
•pRABBIT IgG IRES-EmGFP 阳性对照载体，在 2–8° 下储存或在 -20°C 下长期储存
• 1 L Expi293 表达培养基，在 2–8°C 下储存（避光储存）
• ExpiFectamine 293 转染试剂盒，用于 1 L 培养，在 2–8°C 下储存
• PNGase F 聚糖切割试剂盒，在 -20°C 下储存
• 100 mL Opti-Plex 综合缓冲液，在 2–8°C 下储存（避光）
• 20 µg pcDNA5/TO 哺乳动物表达载体，在 2–8°C 下储存或在 -20°C 下长期储存。
•pRABBIT IgG IRES-EmGFP 阳性对照载体，在 2–8° 下储存或在 -20°C 下长期储存