TMTpro™ 零标记试剂
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Thermo Scientific™

TMTpro™ 零标记试剂

Thermo Scientific TMTpro 零标记试剂作为 MS 级肽定量的“轻”标签用于方法优化，或者与 TMTpro 16plex 标记试剂套件配合使用。TMTpro了解更多信息
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货号数量
A445195 x 0.5 mg
A445181 x 5 mg
货号 A44519
价格（CNY)
2,814.00
Each
添加至购物车
数量:
5 x 0.5 mg
价格（CNY)
2,814.00
Each
添加至购物车

Thermo Scientific TMTpro 零标记试剂作为 MS 级肽定量的“轻”标签用于方法优化，或者与 TMTpro 16plex 标记试剂套件配合使用。

TMTpro 零标记试剂的特点包括：
•经济—轻”版本可用于 TMTpro 标记样品的优化和方法开发
• 高效—胺反应性、NHS 酯活化试剂可确保高效标记所有肽，无需考虑蛋白序列或蛋白水解酶的特异性
• 兼容—经过优化可与高分辨率 Thermo Scientific MS/MS 平台（如 Q Exactive 和 Orbitrap Fusion Tribrid 仪器系列，包括 Orbitrap Eclipse Tribrid 和 Orbitrap Exploris 480 质谱仪）配合使用，并且 Proteome Discoverer 2.3 完全支持数据分析

TMTpro 标记试剂是新一代串联质谱标签，设计用于提高样品多通路水平，同时不影响蛋白鉴别和定量。TMTpro 标签结构与 TMT 标签结构类似，因为标签仍为同量异序和胺反应性，但是在长间隔区和异丁基脯氨酸质量报告基因区不同。在 MS/MS 裂解之后，每个 TMTpro 标签在高分辨率 MS/MS 质谱的低质量区域生成一个唯一的报告质量（例如 TMTpro 126-134Da），用于蛋白质表达水平的相对定量。TMTpro 零标记试剂是 TMTpro 标签的“轻”版本，可用于方法开发，或者与一组“重”TMTpro 试剂或其中部分试剂联合使用。

要对 TMTpro 标记的肽进行高分辨率分析，建议对 Nanospray Flex 源使用 Acclaim PepMap 100 C18 LC 色谱柱（货号：164942 或 164939）。对于 EASY-Spray 离子源，推荐 LC 色谱柱为 EASY-Spray C18 LC 色谱柱（货号 ES802 或 ES803）。

这些产品仅适用于应用范围有限的标签许可。

仅供科研使用。不可用于诊断程序。
规格
描述TMTpro™ 零标记试剂，5 x 0.5 mg
最终产品类型肽段
适用于（设备）质谱仪
标签或染料Tandem Mass Tag™ (TMT) 标记
数量5 x 0.5 mg
运输条件干冰
工作流程步骤蛋白质标记
检测方法质谱分析
产品线TMT™
原始材料肽
Unit SizeEach

常见问题解答 (FAQ)

What is the purpose of the TMTpro Zero Label Reagent?

The TMTpro Zero Label Reagent was designed to be used to optimize methods before multiplexed analysis of samples.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can the TMT and TMTpro reagents be mixed?

No, the TMT and TMTpro reagents cannot be mixed because the reporter ions are in the same region.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can TMTpro label reagents dissolved in anhydrous acetonitrile be dried down and stored for later use?

Yes, unused TMTpro label reagent that has been dissolved in anhydrous acetonitrile can be aliquoted into tubes, dried under vacuum at 25-30 degrees C, purged with nitrogen, and stored in a sealed bag with desiccant at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

At which step should I label my mass spec samples with TMT reagents?

TMT labeling can be performed after reduction, alkylation, digestion, or prior to cleanup, if the sample is buffered at appropriate pH (8-8.5) in a suitable buffer without primary amines (e.g., Tris, glycine). TMT labeling can also be performed after peptide clean-up. Labeling of peptides after clean-up enables measuring and normalizing of the peptide samples for equal mixing.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Do I have to apply correction factors for analyzing data for TMT/TMTpro-labeled samples?

Applying correction factors is required for more accurate relative protein quantitation as the isotopic impurity is variable among the tags, ranging from 5-10%.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all TMTpro™ 零标记试剂 FAQs