Expi293™ Flex Cells

Expi293 Flex Cells represent an advanced HEK293 platform for rapid, high-yield protein expression. With optimized growth characteristics and stable expression performance over 40 passages, they achieve reliable scalability for various protein types and expression formats.

Derived from parental HEK293 cells, Expi293 Flex Cells serve as the core component of the Expi293 Flex Expression System. Cultivated exclusively in suspension, Expi293 Flex cells attain high densities in Expi293 Flex Expression Medium. With high transfectability, the cells deliver markedly higher protein yield than standard HEK293 cell lines for transient protein expression workflows.

Attributes of Expi293 Flex Cells include:

• Suspension culture cells pre-adapted to Expi293 Flex Expression Medium
• Higher protein expression levels than standard HEK 293 cells adapted to Expi293 Flex Expression Medium
• Faster proliferation and improved cell viabilities than standard suspension-adapted HEK 293 cells
• Consistent transient expression performance across up to 40 post-thaw cell passages.

Each vial contains 1 mL frozen cells suspended at 1x10⁷ viable cells/mL in Expi293 Flex Medium and 10% DMSO; cells should be thawed straight into Expi293 Flex Expression medium. The 6-vial multipack avoids in-house cell expansion and rebanking work.

Cells are normally cultured in shake flasks inside humidified CO₂ incubators. The system scales easily from <1 mL multiwell cultures to bioreactor runs over 10 L. Harvest timing ranges from 2 to 7 days after transfection based on target protein, with antibody collection routinely scheduled at Day 4–7 post-tranfection. No supplemental medium or fed-batch feeding is required throughout the expression phase.