Expi293 Flex Expression System Kit

The Gibco Expi293 Flex Expression System is a complete solution for rapid, high-yield protein production from HEK293 cells. It is a fully integrated expression system designed to deliver higher yields of a wider variety of proteins-including challenging and low-yield proteins-with exceptional speed, efficiency, and an easy-to-automate protocol. Producing increasingly complex proteins with higher yields and increased throughput is critical to accelerating breakthroughs in protein research applications and drug development.

The Gibco Expi293 Flex Expression System delivers a comprehensive platform for fast, high-yield protein production using HEK293 cells. Its fully integrated design supports robust expression across broad protein portfolios, including difficult-to-express and low-yield proteins-with remarkably rapid, efficient, readily automatable workflows. Achieving improved protein yields and higher throughput for intricate proteins is essential to expediting advances in protein research and pharmaceutical development pipelines.

The Expi293 Flex Expression System features include:

• Expand your target spectrum by successfully producing difficult-to-express and low-yield proteins.
• Markedly elevated productivity; multi-fold increase in protein yields—up to 3 g/L
• Cost effective; the lower cost/mg
• Rapid production of recombinant proteins with harvest ready in 2–4 days
• Supports high-throughput screening and is fully compatible with automated workflows.
• High density cultures of Expi293 Flex cells that result in more expressing cells per milliliter of culture
• Reduce reagents, plasticware, and labor by 2X
• Native folding and mammalian post-translational modification of expressed proteins
• Scalable expression from <1 mL to >10 L

The Expi293 Flex Expression System Kit consists of cryopreserved Expi293 Flex cells (2 vials), 1 L Expi293 Flex Expression Medium, one Expi293 Flex Transfection Kit qualified for 1 L culture transfection, Opti‐Plex Complexation Buffer, and an antibody-expressing positive control vector. All components of the Expi293 Flex Expression System are also available for individual purchase.