What are the concentrations of the primers and probes for TaqMan microbial assays?
Our predesigned and custom TaqMan microbial 20X assays have a primer concentration of 900 nM and a probe concentration of 250 nM in the final 1X concentration.
Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.
For TaqMan Microbe Detection and Antibiotic Resistance Assays, what do the different banners mean (Qualified, Recommended, Control)?
- A recommended assay is the assay with the best strain coverage based on in silico prediction. In other words, recommended assays have the highest number of predicted on-targets and lowest number of predicted off-targets.
- A qualified assay has gone through R&D testing within the context of our main microbe detection applications, such as urinary tract, respiratory tract, and vaginal microbiota detection.
- A control banner is used exclusively for common control assays used in microbe detection applications.
I would like to order a control for my TaqMan Microbe Detection and Antibiotic Resistance Assay. Do you offer one? Do you have options for a custom control?
We have several controls for our pathogen-related content. To find a list of all available amplification controls, simply search in the Microbe Detection Search Tool (https://www.thermofisher.com/microbe-detection/taqman/query/) and click on the Controls option. Please contact Technical Support if you are unsure whether a particular control is compatible with your assay of interest.
We also have options for creating a custom control, containing your specific targets of interest. You can find more information here:
https://www.thermofisher.com/us/en/home/clinical/clinical-genomics/pathogen-detection-solutions/real-time-pcr-panel-controls.html
What protocol do you recommend using for the TaqMan Microbe Detection and Antibiotic Resistance Assays?
When using the assays in a single-tube format, we recommend following this general protocol (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018536_TaqManMicrobialAssays_SingleTube_QR.pdf).
How do I find the TaqMan Microbe Detection and Antibiotic Resistance Assay I'm looking for and search for multiple targets?
There are several ways to search for TaqMan Microbe Detection and Antibiotic Resistance Assays. From the main Thermo Fisher Scientific website page (www.thermofisher.com), hover over the word Popular in the top navigation bar. From the menu that comes up, select TaqMan Real-Time PCR Assays. Under The right TaqMan Assay is just a couple of clicks away, from the TaqMan Assay dropdown, select Microbe & ABR Detection. In the search box, you can then enter your target organism and find your assay of interest.
You can also use these friendly URLs:
https://www.thermofisher.com/microbe-detection/taqman/query/
https://www.thermofisher.com/antibiotic-resistance/taqman/query/
To search for multiple targets, from the search results displayed above, click on the link in the upper right for Search Multiple Targets.