套装内容胰蛋白酶,2 x 20 µg 胰蛋白酶储存溶液,250 µL 蛋白 A/G 磁珠,1 mL IP-MS 细胞裂解缓冲液,100 mL IP-MS 洗涤缓冲液 A,75 mL IP-MS 洗涤缓冲液 B,40 mL IP 洗脱和 MS 样品制备缓冲液,10 mL 烷基化溶液,1 mL/瓶 低蛋白结合采集管,50 x 1.5 mL Bond-Breaker™ TCEP 溶液,中性 pH 值,0.5 mL 10% Pierce™ 三氟乙酸 (TFA),测序级,0.5 mL 1M MgCl2,0.75 mL
数量20 次反应
工作流程步骤样品制备富集
检测方法质谱分析
产品线SureQuant
原始材料细胞, 组织
Unit SizeEach
内容与储存
将胰蛋白酶储存于 4°C 下。 将胰蛋白酶储存溶液储存于 -20°C 下。
常见问题解答 (FAQ)
Why is the processing time for MS analysis less with SureQuant IP-MS Sample Preparation kits compared to traditional IP methods?
SureQuant Protein IP-MS Sample Preparation kits use a trypsin enzyme elution method to dissociate the bound immune complexes from the magnetic beads, which can then be digested directly in-solution and followed up by MS analysis on the same day; no SDS-PAGE purification is required. The entire process take 4 hours compared to traditional IP methods that usually take around 21 hrs.
What is the difference between SureQuant IP-MS Sample Preparation kits and Pierce MS-Compatible Magnetic IP kits?
SureQuant IP-MS Sample Preparation kits include all reagents necessary for sample lysis, immunoenrichment, elution, digestion, and MS analysis. The entire process is completed in 4 hr for same-day LC-MS/MS analysis as compared to traditional IP methods that usually take around 21 hr. Pierce MS-Compatible Magnetic IP kits provide reagents necessary for the lysis step and the elution step, but not for the digestion step prior to MS analysis.
Can I use SureQuant Protein A/G and SureQuant Streptavidin IP-MS Sample Preparation kits with any sample?
SureQuant IP-MS sample preparation kits have been designed for use with SureQuant quantitation modules for upstream sample preparation for SRM/PRM analysis, but they can be used with any samples where the target protein needs to be enriched by IP or IP-CoIP, prior to MS analysis.