病毒生产细胞 2.0 (CN)
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Gibco™

病毒生产细胞 2.0 (CN)

Gibco 病毒生产细胞 2.0 是从 HEK293F 亲代细胞系获得的克隆细胞系,是 AAV-MAX Helper-Free AAV 生产系统的核心组分了解更多信息
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货号 A52021
价格(CNY)
14,721.00
Each
添加至购物车
价格(CNY)
14,721.00
Each
添加至购物车
Gibco 病毒生产细胞 2.0 是从 HEK293F 亲代细胞系获得的克隆细胞系,是 AAV-MAX Helper-Free AAV 生产系统的核心组分。细胞已适应化学成分明确的病毒生产培养基,并在该培养基中保持悬浮培养。使用用于腺相关病毒 (AAV) 生产的 AAV-MAX 转染试剂盒后,病毒生产细胞 2.0 具有高转染性

病毒生产细胞 2.0 的特点包括:
• 源自 293F 的高产克隆细胞系
• 不含 SV40 大 T 抗原
• 经过优化,可在化学成分确定的培养基中进行高密度悬浮培养(>1200 万个细胞/mL)
• 可产生高 AAV 滴度,大于 5 x 1010 vg/mL(未纯化)
• 强大的可扩展性和传代稳定性

冷冻细胞装在样品瓶中提供,其包含 1 mL 浓度为 1 x 107 个活细胞/mL 的细胞,放在病毒生产培养基以及 10% DMSO 中。细胞应直接解冻至病毒生产培养基中,其中添加了 4 mM GlutaMAX 添加物(货号35050038),以便获得极佳性能。

规格
适用于(应用)AAV 生产
产品规格Vial
推荐培养基Gibco™ 病毒生产培养基
池数量1 x 107 个细胞
产品线AAV-MAX
产品类型病毒生产细胞
数量1 mL
运输条件国内运输使用干冰;国际运输使用液氮
来源
经过测试AAV 生产
细胞系Viral Production Cells 2.0 (CN)
细胞类型肾脏(胚胎)
培养类型悬浮液细胞培养
形式Frozen
种属
Unit SizeEach

常见问题解答 (FAQ)

Can I use Viral Production Cells (Cat. No. A35347, A35827) with the AAV MAX Helper-Free AAV Production System?

The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.

What is the highest density that Viral Production Cells 2.0 can be cultured at?

The Viral Production Cells 2.0 achieve maximum cell densities of >1.2 x 10E7 cells/mL in shake flask cultures.

What is the passage stability of the Viral Production Cells 2.0?

We recommend using the Viral Production Cells 2.0 between passages 3 to 25 for adeno-associated virus (AAV) production.

How long do the Viral Production Cells 2.0 require to recover post-thaw before transfection?

We recommend allowing freshly thawed Viral Production Cells 2.0 to recover in culture for at least three passages prior to transfection.

Are the Viral Production Cells 2.0 a clonal cell line?

Yes, the Viral Production Cells 2.0 cells are a clonal cell line derived from single cell cloning of 293-F cells.