I finished running my experiment on the QuantStudio Absolute Q Digital PCR instrument but just realized that I had selected the wrong dyes during setup. Can I change the dyes that were used after the run?
If dye channels are turned off before the run, then data will not be collected for them as the run progresses. This means that changing the dyes after the run will not be possible.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
I ran my experiment using the QuantStudio Absolute Q Digital PCR System but did not actually use dyes in all the channels. Is there a way to only see those dye channels that were used?
Unused dye channels can be turned off after the run by going to the SETUP tab and clicking EDIT SETUP and then EDIT GROUPS. Here, the Analysis can be changed to “Not Used.” After clicking SAVE twice, the data will be reanalyzed and the unused dye channels will not be present in the analysis.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
I'm using the QuantStudio Absolute Q Digital PCR System and the software is saying that the concentration is NaN. What does that mean?
NaN stands for “not a number”. The software will display NaN instead of an actual number when it has detected some issue during the analysis of the array images.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
With the QuantStudio Absolute Q Digital PCR instrument, the run data didn't automatically transfer after the run completed. How can I get the data?
We would recommend restarting the software. If the run still doesn't transfer, we would recommend closing the software, rebooting the instrument, and then starting the software. If further assistance is needed, please contact Technical Support at techsupport@thermofisher.com.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
With the QuantStudio Absolute Q Digital PCR System, how much sample can be loaded into each well?
9 µL of sample is loaded into each well along with 15 µL of isolation buffer. Loading more or less of either the sample or isolation buffer will affect filling of the microchambers and the run results.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
