Pierce™ 高能力蛋白 A 磁珠,对碱稳定
Pierce™ 高能力蛋白 A 磁珠,对碱稳定
Thermo Scientific™

Pierce™ 高能力蛋白 A 磁珠,对碱稳定

Pierce 高能力蛋白 A 磁珠对碱稳定,是一种高性能磁珠,适用于从细胞培养物上清液、腹水、血清和粗提取物中纯化抗体。高能力蛋白 A 磁珠的特点包括:• 高结合能力—≥了解更多信息
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货号数量
A530365 mL
A530351 mL
A5303725 mL
A530384 x 25 mL
货号 A53036
价格(CNY)
6,180.00
飞享价
Ends: 31-Dec-2025
8,364.00
共减 2,184.00 (26%)
Each
添加至购物车
数量:
5 mL
价格(CNY)
6,180.00
飞享价
Ends: 31-Dec-2025
8,364.00
共减 2,184.00 (26%)
Each
添加至购物车
Pierce 高能力蛋白 A 磁珠对碱稳定,是一种高性能磁珠,适用于从细胞培养物上清液、腹水、血清和粗提取物中纯化抗体。

高能力蛋白 A 磁珠的特点包括:
• 高结合能力—≥ 40 mg HIgG/mL 沉淀微珠
• 对碱稳定 — 经过精心设计以耐受使用 0.1M NaOH 的标准 CIP 程序,从而有效清洁微珠以供重复使用
• 无动物组分原材料
• 多功能性 — 微珠兼容手动和自动化工作流程(例如 KingFisher 仪器)
• 稳定性 — 高亲和力蛋白 A 被牢固固定在磁性琼脂糖上,以防止配体、靶标抗体或碱基载体浸出

蛋白 A 配体经过了基因修饰,在 NaOH 存在的情况下也可保持稳定,因此微珠至少可再生10次且不影响结合能力。该配体在酵母中产生并固定在平均粒径 ∼30 µm 的磁性琼脂糖上。

Pierce 高能力蛋白 A 磁珠对碱稳定,适用于纯化 mAb 以及含有 VH3 结构域的 scFv 和 Fab 片段亚群。该微珠兼容自动化,可用于更高通量的纯化和纯化条件筛选。

仅供科研使用。不可用于诊断程序。
规格
柱床容量1.25 mL
结合功能≥40 mg HIgG/mL 沉淀微珠
最大浓度Slurry: 25%
描述高能力琼脂糖磁珠
适用于(应用)抗体纯化
产品规格瓶装
配基类型对碱稳定的蛋白 A
数量5 mL
容量(公制)5 mL
粒径∼ 30 μm
Unit SizeEach
内容与储存
以 25% 浆液形式提供。

接收后,在 2–8°C 下储存。

常见问题解答 (FAQ)

Can I automate the workflow using Pierce High Capacity Protein A MagBeads, alkali stable?

Yes. A KingFisher Apex protocol for 96-deep well plates is provided on pages 3 and 4 in the instruction manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0026311_Pierce_HiCap_proteinA_MagBeads_UG.pdf).

Using Pierce High Capacity Protein A MagBeads, alkali stable, what downstream assays are compatible with the eluted samples?

Purified samples eluted from Pierce High Capacity Protein A MagBeads, alkali stable are compatible with protein assays (Bradford, BCA, Rapid Gold, A280, etc.) and can be used for industry standard testing applications (western blotting, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, etc.).

After regeneration of Pierce High Capacity Protein A MagBeads, alkali stable, is there a specific buffer in which the beads should be stored?

If the beads are not going to be re-used immediately, we recommend using 20% ethanol for long-term storage.

How do I regenerate Pierce High Capacity Protein A MagBeads, alkali stable?

Using the Clean-In-Place (CIP) protocol provided in the instruction manual, we recommend cleaning the Pierce High Capacity Protein A MagBeads, alkali stable with 20 bed volumes of 0.1 M NaOH after every run.

How many uses/regenerations are possible with Pierce High Capacity Protein A MagBeads, alkali stable?

Pierce High Capacity Protein A MagBeads, alkali stable can be reused at least 10 times with <5% loss in binding capacity.