ATTO-TAG™ CBQCA 衍生化试剂(CBQCA;3-(4-羧基苯甲酰基)喹啉-2-羧醛)
Product Image
Invitrogen™

ATTO-TAG™ CBQCA 衍生化试剂(CBQCA;3-(4-羧基苯甲酰基)喹啉-2-羧醛)

ATTO-TAG™ CBQCA 衍生化试剂(CBQCA;3-(4 羧基苯甲酰基)喹啉-2-羧醛)可与伯胺发生特异性反应,形成可通过电泳或色谱方法进行分析的偶联物。CBQCA 的所得产品在 450 nm了解更多信息
Have Questions?
货号数量
A6222
又称 A-6222
10 mg
货号 A6222
又称 A-6222
价格(CNY)
4,565.00
飞享价
Ends: 31-Dec-2025
5,802.00
共减 1,237.00 (21%)
Each
添加至购物车
数量:
10 mg
请求批量或定制报价
价格(CNY)
4,565.00
飞享价
Ends: 31-Dec-2025
5,802.00
共减 1,237.00 (21%)
Each
添加至购物车
ATTO-TAG™ CBQCA 衍生化试剂(CBQCA;3-(4 羧基苯甲酰基)喹啉-2-羧醛)可与伯胺发生特异性反应,形成可通过电泳或色谱方法进行分析的偶联物。CBQCA 的所得产品在 450 nm 处或通过 He-Cd 激光的 442 nm 光谱线得到最大激发,最大发射波长 ∼550 nm。在毛细管区带电泳 (CZE) 中,检测 ATTO-TAG™ CBQCA 偶联物的灵敏度应在阿托摩尔级范围(10-18 摩尔)内。高灵敏度、无背景和长波长激发性使这些潜在试剂适用于研究人员、诊断和法医应用。
仅供科研使用。不可用于诊断程序。
规格
化学反应性
适用于(应用)细胞活力、增殖和功能;蛋白质电泳和 Western 印迹
标签或染料ATTO-TAG CBQCA
数量10 mg
反应一部分醛类
运输条件室温
靶标伯胺
形式实心
产品线ATTO-TAG
类型衍生化试剂
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

Can CBQCA (3-(4carboxybenzoyl) quinoline- 2-carboxaldehyde) be used to label reducing sugars?

Yes, see the reference: Novotny MV, Sudor J (1993) High-performance capillary electrophoresis of glycoconjugates. Electrophoresis 14:373-389.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can NaCN be used instead of KCN in the CBQCA (3-(4carboxybenzoyl) quinoline- 2-carboxaldehyde) assay?

Yes. Use the same molarity; it is the cyanide group that is required for the reaction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (33)

引用和文献
Abstract
CBQCA assay of primary amine losses during hemodialysis.
Authors:Kirschbaum B
Journal:Clin Chim Acta
PubMed ID:11412827
Nitrogen loss during hemodialysis is an important issue since all steps taken to improve clearance of urea will also increase loss of amino acids and other normally recyclable sources of nitrogen. Most dialyzers exclude albumin and other proteins, which react well with dyes commonly employed to measure protein, but do ... More
Derivatization in capillary electrophoresis.
Authors:Bardelmeijer HA, Lingeman H, de Ruiter C, Underberg WJ
Journal:J Chromatogr A
PubMed ID:9646486
'In recent years capillary electrophoresis (CE) has been developed into a versatile separation technique, next to gas and liquid chromatography (LC), well suited for the determination of a wide variety of e.g., pharmaceutical, biomedical and environmental samples. The main advantages of CE over chromatographic separation techniques are its simplicity and ... More
Capillary electrophoresis with laser-induced fluorescence detection: a sensitive method for monitoring extracellular concentrations of amino acids in the periaqueductal grey matter.
Authors:Bergquist J, Vona MJ, Stiller CO, O'Connor WT, Falkenberg T, Ekman R
Journal:J Neurosci Methods
PubMed ID:8815306
'The use of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for the analysis of microdialysate samples from the periaqueductal grey matter (PAG) of freely moving rats is described. By employing 3-(4-carboxybenzoyl)-2-quinoline-carboxaldehyde (CBQCA) as a derivatization agent, we simultaneously monitored the concentrations of 8 amino acids (arginine, glutamine, valine, gamma-amino-n-butyric acid ... More
Mycothiol biosynthesis and metabolism. Cellular levels of potential intermediates in the biosynthesis and degradation of mycothiol in mycobacterium smegmatis.
Authors:Anderberg SJ, Newton GL, Fahey RC
Journal:J Biol Chem
PubMed ID:9804803
'Mycothiol (MSH; 1-D-myo-inosityl-2-(N-acetyl-L-cysteinyl)amido-2-deoxy-alpha-D- glucop yranoside (AcCys-GlcN-Ins)) is a novel thiol produced at millimolar levels by mycobacteria and other actinomycetes that do not make glutathione. We developed methods to determine the major components of MSH (AcCys, Cys-GlcN, AcCys-GlcN, Cys-GlcN-Ins, GlcN-Ins) in cell extracts. Mycobacterium smegmatis was shown to produce measurable levels ... More
Identification of a recombinant synaptobrevin-thioredoxin fusion protein by capillary zone electrophoresis using laser-induced fluorescence detection.
Authors:Asermely KE, Broomfield CA, Nowakowski J, Courtney BC, Adler M
Journal:J Chromatogr B Biomed Sci Appl
PubMed ID:9271130
'Capillary zone electrophoresis (CZE) was utilized to identify a synaptobrevin-thioredoxin fusion protein (TSB-51). TSB-51 is a substrate for cleavage by botulinum toxin B at the Q(76)-F(77) site. TSB-51 was derivatized with a fluorophore, CBQCA [3-(4-carboxy-benzoyl)-2-quinoline-carboxaldehyde], for 4 h at room temperature. Optimal conditions for CZE separation of the TSB-51-CBQCA complex ... More