Plate warping is causing problems with the robot in my automated processes. How can I solve this?
We recommend Thermo Scientific Armadillo PCR plates which are designed for robotic handling with high rigidity and resistance. Combining the rigidity of a polycarbonate frame with thin walled polypropylene wells, the Armadillo plates provide superior thermal cycling performance under all conditions without warping.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Are Thermo Scientific ABgene plastics endotoxin-free?
They are not certified endotoxin-free. However, their entire production process, from molding to final packaging, is carried out in a Class 100,000 clean room under ISO 9001 guidelines. All Thermo Scientific PCR plastics are certified free from RNase, DNase and human DNA.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Are Thermo Scientific ABgene plastics sterile?
Thermo Scientific Abgene plastics are not typically sterilized as their entire production process, from molding to final packaging, is carried out in a Class 100,000 clean room under ISO 9001 guidelines. All Thermo Scientific PCR plastics are certified free from RNase, DNase and human DNA. In contrast, during typical non-clean room production, plastics are exposed to many contaminants including dust, bacterial cells, and DNA. The plastics are then sterilized to kill bacteria and inactivate RNases and DNases, but sterilization does not remove dust or DNA contamination. The dust particles left behind can inhibit PCR, and the damaged DNA fragments can still act as templates, leading to non-specific amplification.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
What 96-well plates do you recommend for -80 degrees C storage?
Both polypropylene and polystyrene plates can be used to store samples at -80 degrees C and depends on the downstream application. Please note that the sealing method selected is critical for storing at these temperatures and heat sealing is recommended.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
I would like to use the flat ultra-clear and domed cap strips for Thermo-Fast PCR Plate, 48-well, clear (Cat. No. AB0648). What do you recommend?
For the domed cap strips, you could choose the Domed PCR Caps, strips of 8 (Cat. No. AB0265). For the flat ultra clear cap strips you can choose for the flat ultra clear cap strips the (Cat. No. AB0866). Generally speaking, within the Thermo Scientific/ABgene legacy, all our cap strips are compatible with all PCR plates and tube strips. For more options, please look at the following link.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.
