4X Bolt™ LDS 上样缓冲液
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4X Bolt™ LDS 上样缓冲液
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4X Bolt™ LDS 上样缓冲液

Bolt LDS 上样缓冲液 (4X) 用于为使用 Bis-Tris Plus 凝胶的变性凝胶电泳制备蛋白样品。其中含有十二烷基磺酸锂 (pH了解更多信息
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货号数量
B0008250 mL
B000710 mL
货号 B0008
价格(CNY)
2,028.00
Each
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数量:
250 mL
价格(CNY)
2,028.00
Each
添加至购物车
Bolt LDS 上样缓冲液 (4X) 用于为使用 Bis-Tris Plus 凝胶的变性凝胶电泳制备蛋白样品。其中含有十二烷基磺酸锂 (pH 8.4),可最大限度提高还原剂活性。

查看 SDS-PAGE 可用的所有缓冲液和试剂

Bolt LDS 上样缓冲液所含示踪剂染料为考马斯 G250 和酚红,而非溴酚蓝。考马斯 G250 在 MES 和 MOPS SDS 电泳缓冲液中均可显示出清晰的染料前沿,并且比溴酚蓝更靠近移动的离子迁移前沿。采用 MES SDS 电泳缓冲液时,溴酚蓝的迁移速度比部分肽段更慢。这可确保小尺寸肽段不会在凝胶上迁移。

使用方法:将 1X 稀释的样品(还原或未还原)在 70°C 下加热 10 分钟以获得理想结果。

注:Bolt LDS 上样缓冲液应在使用前恢复到室温 (25°C)。其未高粘度、浓缩型溶液,含有相当于常用样品缓冲液两倍的 LDS 含量。Bolt LDS 上样缓冲液还含有浓度较高的甘油。
仅供科研使用。不可用于诊断程序。
规格
缓冲液上样缓冲液
最大浓度4X
凝胶兼容性Bolt™ Bis-Tris Plus 预制胶
产品线Bolt™
产品类型LDS 样品缓冲液
数量250 mL
适用于(设备)螺栓
凝胶类型Bis-Tris Plus
Unit SizeEach
内容与储存
上样缓冲液 (4X) 含有十二烷基硫酸锂 (LDS, pH 8.5)、SERVA Blue G250和酚红。

4°C 至 25°C 储存。

常见问题解答 (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.