BacLight™ RedoxSensor™ CTC 活力试剂盒

引用和文献 (6)

Invitrogen™

BacLight™ RedoxSensor™ CTC 活力试剂盒

The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation了解更多信息

货号	数量
B34956	1 Kit

货号 B34956

价格（CNY)

6,616.00

Ends: 31-Dec-2025

8,790.00

共减 2,174.00 (25%)

添加至购物车

1 Kit

价格（CNY)

6,616.00

Ends: 31-Dec-2025

8,790.00

共减 2,174.00 (25%)

添加至购物车

The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product. Cells not respiring or respiring at slower rates will reduce less CTC and consequently produce less fluorescent product, giving a semi-quantitative estimate of healthy vs. unhealthy bacteria. Green- and blue-fluorescent nucleic acid stains are included as counterstains to assist differentiation of cells from debris and calculating total cell numbers.

仅供科研使用。不可用于诊断程序。

细胞类型细菌

检测方法荧光

染料类型其他标记或染料

产品规格管、玻片

数量1 Kit

运输条件室温

溶解度DMSO（二甲亚砜）

发射UV，488

适用于（设备）荧光显微镜, 流式细胞仪

产品线BacLight、RedoxSensor

产品类型CTC 细胞活力试剂盒

Unit SizeEach

内容与储存

含 5 瓶 5-氰基-2,3-二甲苯基四唑氯化物（CTC，每瓶 15 mg）、1 瓶 SYTO™ 24 Green（100 µL，溶于 DMSO）m 和 DAPI（100 µL，5 mg/mL 溶液，溶于水）。在冷柜（-5 至 -30°C）中避光储存

常见问题解答 (FAQ)

我可以在流式细胞仪上看到哪些细菌参数?

您可以用BacLight Green细菌染色剂（货号B35000）或BacLight Red细菌染色剂（货号B35001）等常规染色剂进行细菌染色。你们可以看到革兰氏特性（货号L7005），细胞存活率（货号L7007、L7012和L13152），细胞计数（货号L34856和B7277）和细胞活力。细胞活力可以通过膜电位法（货号B34950）或代谢法（货号B34954和B34956）测定。

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (6)

引用和文献

Abstract

Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.

Authors:Sieracki ME, Cucci TL, Nicinski J,

Journal:Appl Environ Microbiol

PubMed ID:10347021

'The respiratory activity of marine bacteria is an important indication of the ecological functioning of these organisms in marine ecosystems. The redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is reduced intracellularly in respiring cells to an insoluble, fluorescent precipitate. This product is detectable and quantifiable by flow cytometry in individual cells. ... More

Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm.

Authors:Kim J, Pitts B, Stewart PS, Camper A, Yoon J,

Journal:Antimicrob Agents Chemother

PubMed ID:18195062

'The systematic understanding of how various antimicrobial agents are involved in controlling biofilms is essential in order to establish an effective strategy for biofilm control, since many antimicrobial agents are effective against planktonic cells but are ineffective when they are used against the same bacteria growing in a biofilm state. ... More

Characterization of osmotically induced filaments of Salmonella enterica.

Authors:Pratt ZL, Chen B, Czuprynski CJ, Wong AC, Kaspar CW,

Journal:Appl Environ Microbiol

PubMed ID:22798362

'Salmonella enterica forms aseptate filaments with multiple nucleoids when cultured in hyperosmotic conditions. These osmotic-induced filaments are viable and form single colonies on agar plates even though they contain multiple genomes and have the potential to divide into multiple daughter cells. Introducing filaments that are formed during osmotic stress into ... More

Inactivation of Escherichia coli by nanoparticulate zerovalent iron and ferrous ion.

Authors:Kim JY, Park HJ, Lee C, Nelson KL, Sedlak DL, Yoon J,

Journal:Appl Environ Microbiol

PubMed ID:20870787

'The mechanism of Escherichia coli inactivation by nanoparticulate zerovalent iron (nZVI) and Fe(II) was investigated using reactive oxygen species (ROS) quenchers and probes, an oxidative stress assay, and microscopic observations. Disruption of cell membrane integrity and respiratory activity was observed under deaerated conditions [more disruption by nZVI than Fe(II)], and ... More

How the insect immune system interacts with an obligate symbiotic bacterium.

Authors:Douglas AE, Bouvaine S, Russell RR,

Journal:Proc Biol Sci

PubMed ID:20719775

The animal immune system provides defence against microbial infection, and the evolution of certain animal-microbial symbioses is predicted to involve adaptive changes in the host immune system to accommodate the microbial partner. For example, the reduced humoral immune system in the pea aphid Acyrthosiphon pisum, including an apparently non-functional immune ... More

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