DiBAC4(3)（双(1,3-二巴比妥酸)-三次甲基氧烯洛尔）
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DiBAC<sub>4</sub>(3)（双(1,3-二巴比妥酸)-三次甲基氧烯洛尔）
引用和文献 (160)
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DiBAC4(3)（双(1,3-二巴比妥酸)-三次甲基氧烯洛尔）

DiBAC4(3) 是缓慢响应的电位敏感型探针，可以进入去极化细胞，并在此与胞内蛋白或膜结合，呈现出增强的荧光和红色光谱迁移。增强去极化效果，可增加阴离子染料通量进而提升荧光强度。而超极化则表现为荧光强度下降。这种 bis-oxonal 的最大激发波长为490 nm，最大发射波长为516 nm。由于整体带负电荷，DiBAC了解更多信息
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货号数量
B43825 mg
货号 B438
价格（CNY)
3,708.00
飞享价
Ends: 31-Dec-2025
5,027.00
共减 1,319.00 (26%)
Each
添加至购物车
数量:
25 mg
价格（CNY)
3,708.00
飞享价
Ends: 31-Dec-2025
5,027.00
共减 1,319.00 (26%)
Each
添加至购物车
DiBAC4(3) 是缓慢响应的电位敏感型探针，可以进入去极化细胞，并在此与胞内蛋白或膜结合，呈现出增强的荧光和红色光谱迁移。增强去极化效果，可增加阴离子染料通量进而提升荧光强度。而超极化则表现为荧光强度下降。这种 bis-oxonal 的最大激发波长为490 nm，最大发射波长为516 nm。由于整体带负电荷，DiBAC 染料会被线粒体排阻，因而比碳氰酸酯更适合测定细胞膜电位。
仅供科研使用。不可用于诊断程序。
规格
染料类型膜电位探针
激发/发射493/516 nm
分子量516.64
数量25 mg
运输条件室温
产品类型DiBAC4(3)
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

当我使用膜电位指示剂时，看到神经元周围出现了较高的背景，如何降低背景干扰？

如果使用我们的FluoVolt 膜电势试剂盒（货号F10488），该试剂盒包含一种背景抑制剂，可改善这一问题。对于其他指标剂，可以考虑使用BackDrop 背景抑制剂（货号R37603、B10511和B10512）。

快反应膜电位探针和慢反应膜电位探针有什么区别？

在周围电场的作用下结构变化的分子可用作检测瞬时（毫秒级）电位变化的快反应探针。慢反应染料则会进入去极化细胞，结合蛋白或膜。增强去极化会造成额外的染料流入，增强荧光强度；过极化的特征则是荧光强度下降。快反应探针通常用于完整心脏组织的电位活动成像，或测量药理刺激引起的膜电位变化。慢反应探针常用于探索线粒体功能和细胞活力。

你们提供哪些类型的膜电位指示剂？我该如何根据自己的试验选择？

膜电位指示剂选择指南请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html）。

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all DiBAC4(3)（双(1,3-二巴比妥酸)-三次甲基氧烯洛尔） FAQs

引用和文献 (160)

引用和文献
Abstract
A novel membrane potential-sensitive fluorescent dye improves cell-based assays for ion channels.
Authors:Baxter DF, Kirk M, Garcia AF, Raimondi A, Holmqvist MH, Flint KK, Bojanic D, Distefano PS, Curtis R, Xie Y
Journal:J Biomol Screen
PubMed ID:11897058
The study of ion channel-mediated changes in membrane potential using the conventional bisoxonol fluorescent dye DiBAC(4)(3) has several limitations, including a slow onset of response and multistep preparation, that limit both the fidelity of the results and the throughput of membrane potential assays. Here, we report the characterization of the ... More
Characterization of human urinary bladder KATP channels containing SUR2B splice variants expressed in L-cells.
Authors:Scott VE, Davis-Taber RA, Silvia C, Hoogenboom L, Choi W, Kroeger P, Whiteaker KL, Gopalakrishnan M
Journal:Eur J Pharmacol
PubMed ID:14729107
The molecular properties of the sulfonylurea receptor 2 (SUR2) subunits of K(ATP) channels expressed in urinary bladder were assessed by polymerase chain reaction (PCR). This showed that SUR2B exon 17- mRNA (72%) was predominant over the SUR2B exon 17+ splice variant (28%). The pharmacological properties of both of these isoforms ... More
Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.
Authors:Silva E, Gomes P, Soares-da-Silva P,
Journal:J Membr Biol
PubMed ID:17334838
'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More
TRPC1 associates with BK(Ca) channel to form a signal complex in vascular smooth muscle cells.
Authors:Kwan HY, Shen B, Ma X, Kwok YC, Huang Y, Man YB, Yu S, Yao X,
Journal:Circ Res
PubMed ID:19168436
'TRPC1 (transient receptor potential canonical 1) is a Ca(2+)-permeable cation channel involved in diverse physiological function. TRPC1 may associate with other proteins to form a signaling complex, which is crucial for channel function. In the present study, we investigated the interaction between TRPC1 and large conductance Ca(2+)-sensitive K(+) channel (BK(Ca)). ... More
Lipid factor (bVLF) from bovine vitreous body evokes in EGFR-T17 cells a Ca2+-dependent K+ current associated with inositol 1,4,5-trisphosphate-independent Ca2+ mobilization.
Authors:Camiña JP, Diaz-Rodriguez E, Harks EG, Theuvenet AP, Ypey DL, Casanueva FF
Journal:J Cell Physiol
PubMed ID:12599214
'Bovine vitreous lipid factor (bVLF) is a complex phospholipid isolated from bovine vitreous body with strong Ca(2+)-mobilizing activity. In this study, the effects of bVLF on membrane potential were investigated in EGFR-T17 fibroblasts with the whole-cell patch clamp technique on monolayer cells, as well as with the fluorescent dye bis-oxonol ... More
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