CellLight™ 微管蛋白-GFP (BacMam 2.0)

引用和文献 (9)

Invitrogen™

CellLight™ 微管蛋白-GFP (BacMam 2.0)

CellLight™ 微管蛋白-GFP (BacMam 2.0) 可采用绿色荧光蛋白 (GFP) 轻松标记活细胞中的微管蛋白。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。想要标记其他细胞结构？了解有关 CellLight™了解更多信息

货号	数量	靶标
C10613	1 瓶	细胞骨架、微管蛋白

货号 C10613

价格（CNY)

8,521.00

添加至购物车

细胞骨架、微管蛋白

价格（CNY)

8,521.00

添加至购物车

CellLight™ 微管蛋白-GFP (BacMam 2.0) 可采用绿色荧光蛋白 (GFP) 轻松标记活细胞中的微管蛋白。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。

想要标记其他细胞结构？了解有关 CellLight™ 荧光蛋白标记工具的更多信息

这种即用型构建体利用 BacMam 2.0 技术转染进细胞，并在此表达融合至人微管蛋白的 GFP。您可以在几乎无细胞毒性的情况下观察活细胞内的微管蛋白-GFP 行为，并可采用多种追踪和示踪染料进行标记，以便对动态细胞过程进行成像。

表达 CellLight™ 构建体的细胞也可使用甲醛固定，以便使用免疫细胞化学技术进行多通路成像。

CellLight™ 技术特性：
•快速便捷：只需将 CellLight™ 试剂加入细胞，过夜孵育，随后即可成像—或冷冻储存，以便后续实验直接使用
• 高效：转导率高达 90%，适用于多种哺乳动物细胞系，包括原代细胞、干细胞和神经元
• 灵活：在多重分析实验或共定位研究中，可共转导多种 BacMam 试剂；通过简单调整剂量即可严格控制表达水平
• 低毒性：CellLight™ 试剂不会在哺乳动物细胞中复制，适用于生物安全等级 (BSL) 1 处理

BacMam 技术
CellLight™ 微管蛋白-GFP (BacMam 2.0) 是人微管蛋白和 emGFP 的融合构建体，可准确和特异性靶向细胞微管蛋白-GFP。这种融合构建体包装在昆虫病毒杆状病毒中，不会在人细胞中复制，在大多数实验室中被指定为生物安全等级 (BSL) 1，可安全使用。BacMam 技术可高效、超低毒性转导/转染大多数哺乳动物细胞类型。这种瞬时转染检测可从过夜孵育后持续最多五天 — 足以完成大部分动态细胞分析。与所有转染/转导技术一样，BacMam 方法无法以同等效率转染/转导所有细胞，因此不太适合细胞群研究或自动成像/计数。CellLight™ 试剂非常适合细胞或亚细胞共定位实验，以及需要特殊分辨率的细胞功能研究。

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型GFP (EmGFP)

发射可见光

激发波长范围488⁄510

适用于（设备）共聚焦显微镜、荧光显微镜

产品线CellLight

数量1 瓶

运输条件湿冰

靶标细胞骨架、微管蛋白

技术荧光强度

标签类型荧光蛋白

产品类型微管蛋白Tubulin

亚细胞定位微管蛋白、细胞骨架, Tubulin

Unit SizeEach

内容与储存

在 2°C 至 6°C 下避光储存。切勿冷冻。

常见问题解答 (FAQ)

我采用CellLight 标记试剂处理神经元时，转导效率很低，该如何提高转染效率？

与许多其它细胞相比，神经元转导更为困难。提高转导效率的主要方法是采用更多数量的病毒颗粒标记细胞。对于原代神经元，在铺盘时转导要比已培养细胞转导效果更好。此外，蛋白的表达在神经元中发生较慢，表达高峰通常发生于2-3天后而非转导后16小时。

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there any way to preserve the CellLights labeling beyond 5 days?

Cells transduced with the CellLights reagents can be stored frozen for several months after transduction, without loss of expression.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the CellLights products toxic to cells?

If the viral particles are used at the level we recommend, they are very well tolerated by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For how long will the CellLights products label my cells?

The BacMam 2.0 CellLights typically express for 5 days after transduction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellLight™ 微管蛋白-GFP (BacMam 2.0) FAQs

引用和文献 (9)

引用和文献

Abstract

Particles on the move: intracellular trafficking and asymmetric mitotic partitioning of nanoporous polymer particles.

Authors:Yan Y, Lai ZW, Goode RJ, Cui J, Bacic T, Kamphuis MM, Nice EC, Caruso F,

Journal:

PubMed ID:23713907

'Nanoporous polymer particles (NPPs) prepared by mesoporous silica templating show promise as a new class of versatile drug/gene delivery vehicles owning to their high payload capacity, functionality, and responsiveness. Understanding the cellular dynamics of such particles, including uptake, intracellular trafficking, and distribution, is an important requirement for their development as ... More

Advanced laboratory techniques for sample processing and immunolabeling using microwave radiation.

Authors:Ferris AM, Giberson RT, Sanders MA, Day JR,

Journal:J Neurosci Methods

PubMed ID:19520116

A better understanding of improved microwave technology has increased the benefits and versatility of the technique as it applies to all aspects of immunohistochemistry. The role of continuous magnetron power output (wattage) combined with precise control of sample heating demonstrated their significance to complex labeling protocols. Here, we present results ... More

Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function.

Authors:Jaqaman K, Kuwata H, Touret N, Collins R, Trimble WS, Danuser G, Grinstein S,

Journal:Cell

PubMed ID:21854984

The mechanisms that govern receptor coalescence into functional clusters--often a critical step in their stimulation by ligand--are poorly understood. We used single-molecule tracking to investigate the dynamics of CD36, a clustering-responsive receptor that mediates oxidized LDL uptake by macrophages. We found that CD36 motion in the membrane was spatially structured ... More

Spreading of neurodegenerative pathology via neuron-to-neuron transmission of ß-amyloid.

Authors:Nath S, Agholme L, Kurudenkandy FR, Granseth B, Marcusson J, Hallbeck M,

Journal:J Neurosci

PubMed ID:22745479

Alzheimer's disease (AD) is the major cause of dementia. During the development of AD, neurofibrillary tangles progress in a fixed pattern, starting in the transentorhinal cortex followed by the hippocampus and cortical areas. In contrast, the deposition of ß-amyloid (Aß) plaques, which are the other histological hallmark of AD, does ... More

Baculovirus-mediated gene transfer into mammalian cells.

Authors:Boyce FM, Bucher NL,

Journal:Proc Natl Acad Sci U S A

PubMed ID:8637876

This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More

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