Gibco™ BASIC MEM

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.
MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. This MEM contains Earle’s salts for use in a CO₂ incubator. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g ⁄ L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Gibco® 'BASIC' products offer value-minded scientists liquid cell culture media and buffer solutions at a significant price savings. As part of Life Technologies’ commitment to quality, this select range of Research Use Only products is continually tested to ensure performance and customer satisfaction.