Ultrahigh resolution imaging of biomolecules by fluorescence photoactivation localization microscopy.
Authors:Hess ST, Gould TJ, Gunewardene M, Bewersdorf J, Mason MD,
Journal:Methods Mol Biol
PubMed ID:19488720
Diffraction limits the biological structures that can be imaged by normal light microscopy. However, recently developed techniques are breaking the limits that diffraction poses and allowing imaging of biological samples at the molecular length scale. Fluorescence photoactivation localization microscopy (FPALM) and related methods can now image molecular distributions in fixed ... More