CountBright™ 绝对计数微珠，用于流式细胞分析
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CountBright™ 绝对计数微珠，用于流式细胞分析
引用和文献 (7)
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CountBright™ 绝对计数微珠，用于流式细胞分析

CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation了解更多信息
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货号数量
C369505 mL
货号 C36950
价格（CNY)
3,860.00
Online Exclusive
Ends: 31-Dec-2025
5,078.00
共减 1,218.00 (24%)
Each
添加至购物车
数量:
5 mL
价格（CNY)
3,860.00
Online Exclusive
Ends: 31-Dec-2025
5,078.00
共减 1,218.00 (24%)
Each
添加至购物车
CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolute cell counts using this single-platform method are more accurate and less complicated than cell concentration determined using multiple-platform testing. CountBright™ absolute counting beads can be used with any sample type, including no-wash/lysed whole blood.

View information about all flow cytometry cell counting beads.
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
直径（公制）7 μm
发射任意激光（UV 至 633/635）
激发波长范围紫外至 635/385 至 800 nm
适用于（设备）流式细胞仪
产品规格溶液
测试数量100
数量5 mL
运输条件室温
产品线CountBright
产品类型细胞计数微珠
Unit SizeEach
内容与储存
包含 1 瓶 CountBright™ 绝对计数微珠 (5 mL)。在冰箱（2°C 至 8°C）中避光储存。

常见问题解答 (FAQ)

使用Live/Dead BacLight细菌活力和计数试剂盒进行流式细胞术实验时，部分细胞好像同时出现红绿两种信号，这些细胞是已经死亡、仍在存活还是即将死亡？

试剂盒中的绿色染料是细胞通透性核酸染色剂，会标记所有细胞。红色染料不能渗透进细胞，只能对膜受损的细胞（死细胞）进行染色。因此，任何具有红色信号的细胞会被认为是死亡的。您的细胞可能会出现有些只有红色信号，有些有红色和绿色信号，有些只有绿色信号的现象。有时红色染料会置换绿色染料。在任何情况下，红色细胞都是死亡的。

另外，绿色染料可能漏进红色通道。进行单色染色，同时在绿色和红色滤光片下检查确定漏光程度。为了避免这种漏光的情况，请使用低浓度的染料；如果可能的话，使用窄带通滤光片。

如何通过流式细胞术对细菌进行计数？

这里有几种办法。我们有两种荧光试剂盒可用于细菌计数：Live/Dead BacLight Bacterial Viability and Counting Kit（货号L34856）；Bacteria Counting kit, for flow cytometry（货号B7277）。另一个选择是Flow Cytometry Sub-micron Particle Size Reference Kit（货号F13839）。

我不太清楚CountBright绝对计数微珠（货号C36950）实验方案中的公式，你们可以帮助我吗？

该公式是让您用计数区域的细胞数除以分析的微珠数。该值随后乘以你们加入的微珠数。该试验方案要求加入50 µL微珠，并且微珠的包装瓶上列出了每50 µL所含的微珠数量 - 你们要乘以该数值（不要除以50）。最后，将得到的结果除以1000即是细胞数/µL。

我用计数微珠进行细胞计数，但在散点图上找不到微珠，该怎么办？

首先检查您的阈值，看它是否设置为前向散射。如果是的话，微珠可能被阈值排除。降低阙值设置后，应该就可以显示出您的微珠了。

我想用流式细胞仪计数细胞，我该怎么做？

可以向样品中添加内部微球计数的标准品来完成流式细胞仪细胞计数。收集到的微球数量已知体积。这使得您可以计算细胞浓度。

View all CountBright™ 绝对计数微珠，用于流式细胞分析 FAQs

引用和文献 (7)

引用和文献
Abstract
An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.
Authors:Trinidad EM, Ballesteros M, Zuloaga J, Zapata A, Alonso-Colmenar LM,
Journal:Blood
PubMed ID:19828693
'Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed ... More
Analysis of microRNA and protein transfer by exosomes during an immune synapse.
Authors:Villarroya-Beltri C, Gutiérrez-Vázquez C, Sánchez-Madrid F, Mittelbrunn M,
Journal:
PubMed ID:23719941
'Immune cells release microRNA-containing exosomes that can be taken up by recipient cells. Exosomes can thus act as mediators of cell-cell communication through direct exchange of genetic material between cells. Exosome-mediated transfer of miRNAs between T cells and antigen-presenting cells (APCs) can take place over long distances. Our work has ... More
Inhibition of NF-?B-mediated signaling by the cyclin-dependent kinase inhibitor CR8 overcomes prosurvival stimuli to induce apoptosis in chronic lymphocytic leukemia cells.
Authors:Cosimo E, McCaig AM, Carter-Brzezinski LJ, Wheadon H, Leach MT, Le Ster K, Berthou C, Durieu E, Oumata N, Galons H, Meijer L, Michie AM,
Journal:Clin Cancer Res
PubMed ID:23532892
Chronic lymphocytic leukemia (CLL) is currently incurable with standard chemotherapeutic agents, highlighting the need for novel therapies. Overcoming proliferative and cytoprotective signals generated within the microenvironment of lymphoid organs is essential for limiting CLL progression and ultimately developing a cure. We assessed the potency of cyclin-dependent kinase (CDK) inhibitor CR8, ... More
PKCa negatively regulates in vitro proplatelet formation and in vivo platelet production in mice.
Authors:Williams CM, Harper MT, Poole AW,
Journal:Platelets
PubMed ID:23402219
Proplatelet formation is a part of the intricate process by which platelets are generated by their precursor cell, the megakaryocyte. The processes that drive megakaryocyte maturation and platelet production are however still not well understood. The protein kinase C (PKC) family of serine/threonine kinases has been demonstrated as an important ... More
CD26 inhibition enhances perfusion recovery in ApoE-/-mice.
Authors:Haverslag RT, de Groot D, Grundmann S, Meder B, Goumans MJ, Pasterkamp G, Hoefer IE, de Kleijn DP,
Journal:Curr Vasc Pharmacol
PubMed ID:23391419
The adaptive growth of blood vessels is important to prevent tissue loss following arterial occlusion. Extravasation of monocytes is essential for this process. The peptidase CD26 targets SDF-1 alpha, a chemokine regulating monocyte trafficking. We hypothesized that blocking SDF-1 alpha inactivation, using a commercially available CD26 inhibitor, accelerates perfusion recovery ... More
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