Cascade Blue™ 酰肼，三钠盐
Cascade Blue™ 酰肼，三钠盐
引用和文献 (24)
Invitrogen™

Cascade Blue™ 酰肼，三钠盐

可固定的细胞不可透过性、极性示踪剂 Cascade Blue™ 酰肼通常用于研究细胞-细胞和细胞-脂质体融合以及膜可透过性和胞饮过程中通过间隙连接或细胞摄取进行的转运。该水溶性示踪剂可通过全细胞膜片钳、离子电渗、胞饮囊泡的渗透裂解或类似方法进入细胞。此外，蛋白和其他水溶性生物聚合物的羧酸可以使用水溶性碳二亚胺（如了解更多信息
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货号数量
C68710 mg
货号 C687
价格（CNY)
4,697.00
Each
添加至购物车
数量:
10 mg
价格（CNY)
4,697.00
Each
添加至购物车
可固定的细胞不可透过性、极性示踪剂 Cascade Blue™ 酰肼通常用于研究细胞-细胞和细胞-脂质体融合以及膜可透过性和胞饮过程中通过间隙连接或细胞摄取进行的转运。该水溶性示踪剂可通过全细胞膜片钳、离子电渗、胞饮囊泡的渗透裂解或类似方法进入细胞。此外，蛋白和其他水溶性生物聚合物的羧酸可以使用水溶性碳二亚胺（如 EDAC (E2247)）在水溶液中与这种分子偶联。
仅供科研使用。不可用于诊断程序。
规格
化学反应性羧酸、酮、醛
发射412 nm
激发399 nm
标签或染料Cascade Blue™
分子量596.44 G/MOL
产品类型酰肼
数量10 mg
反应一部分胺、酰肼
运输条件室温
标签类型经典染料
产品线CASCADE BLUE
Unit SizeEach
内容与储存
室温避光储存。

常见问题解答 (FAQ)

What is the recommended concentration for preparation of Cascade Blue hydrazide, Trisodium Salt (Cat. No. C687) stock solution?

We recommend preparing a 1% w/v stock solution in sterile water. Please note that the dye can aggregate in solution over time.

引用和文献 (24)

引用和文献
Abstract
Parathyroid hormone controls the size of the intracellular Ca(2+) stores available to receptors linked to inositol trisphosphate formation.
Authors:Short AD, Taylor CW
Journal:J Biol Chem
PubMed ID:10636879
'In HEK 293 cells stably expressing type 1 parathyroid (PTH) receptors, PTH stimulated release of intracellular Ca(2+) stores in only 27% of cells, whereas 96% of cells responded to carbachol. However, in almost all cells PTH potentiated the response to carbachol by about 3-fold. Responses to carbachol did not desensitize, ... More
Neuropeptides exert direct effects on rat thymic epithelial cells in culture.
Authors:Head GM, Mentlein R, von Patay B, Downing JE, Kendall MD
Journal:Dev Immunol
PubMed ID:9716910
'To determine if major thymic neuropeptides and neurotransmitters can directly influence the functional activity of cultured rat thymic epithelium, neuropeptides and neurotransmitters were applied, and intercellular communication, proliferation, and thymulin secretion assessed. After injections of a mixture of lucifer yellow dextran (too large to pass gap junctions) and cascade blue ... More
Oligosaccharide organization of the beta-subunits of pig kidney Na+/K+-ATPase.
Authors:Amler E, Staffolani R, Baranska J, Obsil T, Urbanová P, Bertoli E, Mazzanti L
Journal:Physiol Res
PubMed ID:9730046
'The distance between the beta-subunits of Na+/K+-ATPase isolated from pig dark red kidney medulla was determined by Förster energy transfer. First, oligosaccharides of the beta-subunit were shown to be labelled with three fluorophores: Lucifer yellow (LY), Lissamine rhodamine B sulfonyl hydrazine (LRSH) and Cascade blue (CB). Further, LY and LRSH ... More
Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine.
Authors:Geoghegan KF, Stroh JG
Journal:Bioconjug Chem
PubMed ID:1325188
'The 2-amino alcohol structure -CH(NH2)CH(OH)- exists in proteins and peptides in N-terminal Ser or Thr and in hydroxylysine. Its very rapid oxidation by periodate at pH 7 generates an aldehyde in the peptide and is the first step in a method for site-directed labeling with biotin or a fluorescent reporter. ... More
Functional analysis of amino acid sequences in connexin43 involved in intercellular communication through gap junctions.
Authors:Becker DL, Evans WH, Green CR, Warner A
Journal:J Cell Sci
PubMed ID:7615666
'Gap junctions allow direct communication between cells without recourse to the extracellular space and have been widely implicated as important mediators of cell-cell signalling. They are constructed from the connexin proteins, which form a large family, and individual connexins show complex spatial and temporal variations in their expression patterns. Understanding ... More
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