CyQUANT™ NF 细胞增殖检测
CyQUANT™ NF 细胞增殖检测
引用和文献 (5)
Invitrogen™

CyQUANT™ NF 细胞增殖检测

CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。CyQUANT™ NF 细胞增殖检测的特点为：•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏•线性检测范围为每孔100至20,000个细胞了解更多信息
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货号细胞类型数量
C7027Cell Lysis Buffer50 mL
C35011Direct Cell10 Microplates
C35013Direct Red Cell10块微孔板
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C7026For cells in culture1000 次检测
C35006NF Cell1000 Assays
货号 C7027
价格（CNY)
2,264.00
Each
添加至购物车
细胞类型:
Cell Lysis Buffer
数量:
50 mL
价格（CNY)
2,264.00
Each
添加至购物车
CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。

CyQUANT™ NF 细胞增殖检测的特点为：

•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏
•线性检测范围为每孔100至20,000个细胞（96孔微孔板）
•测定试剂盒可以在一小时内完成

快速简单的细胞增殖检测方法
CyQUANT™ NF 细胞增殖测定试剂盒不需要细胞裂解、长时间孵育、放射性标记或从细胞中去除染色剂。CyQUANT™ NF 检测无需进行原始 CyQUANT™ 细胞增殖检测的冻融细胞裂解步骤，代之以一种细胞通透性的 DNA 结合染料和一种质膜通透性的试剂。CyQUANT™ NF 细胞增殖测定试剂盒可以在96孔或384孔微孔板内使用，有两种包装形式：200次测定试剂盒 (C35007)，适用于较小样本量，以及1000次测定试剂盒 (C35006)，适用于高通量应用。

AlamarBlue™ 是 TREK Diagnostic Systems 公司的注册商标。
仅供科研使用。不可用于诊断程序。
规格
细胞类型Cell Lysis Buffer
描述CyQUANT™ 细胞裂解缓冲液 (20X)
染料类型Other Labels or Dyes
形式液体
数量50 mL
试剂类型细胞裂解缓冲液
运输条件室温
适用于（应用）检测
适用于（设备）微孔板读数仪
产品线CyQUANT
产品类型缓冲液
Unit SizeEach
内容与储存
在室温下储存。

常见问题解答 (FAQ)

Is the ATP Determination Kit suitable for frozen heart tissue, tissue homogenate, or serum or plasma (Cat. No. A22066)?

Yes, the ATP Determination Kit (Cat. No. A22066) can be used for tissue samples like frozen heart tissue, as well as blood serum and plasma samples.

Please note, you must physically homogenize the tissue and then lyse the cells using a low-detergent lysis buffer.

We offer the following low-detergent lysis buffer:

Pierce Luciferase Cell Lysis Buffer (2X) (Cat. No. 16189)

As an alternative, you can make your own 20X buffer using this recipe:

20X Cell lysis buffer:
200 mM Tris, pH 7.5
2 M NaCl
20 mM EDTA
0.2 % Triton X-100

Reference:

Ahn BH, Kim HS, Song S, Lee IH, Liu J, Vassilopoulos A, Deng CX, Finkel T. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14447-52. doi: 10.1073/pnas.0803790105. Epub 2008 Sep 15. PMID: 18794531; PMCID: PMC2567183.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (5)

引用和文献
Abstract
Detection of residual donor leucocytes in leucoreduced red blood cell components using a fluorescence microplate assay.
Authors:Gilbert RL, Rider JR, Turton JR, Pamphilon DH
Journal:J Immunol Methods
PubMed ID:12609529
'In November 1999, universal leucoreduction of blood components was introduced in the UK to minimise the risk of variant Creutzfeldt-Jakob Disease (vCJD) transmission by blood transfusion. The UK specifications for leucodepletion processes state that 99% of leucodepleted components should contain < 5 x 10(6) leucocytes/unit, within 95% confidence limits. However, ... More
AMPK and Endothelial Nitric Oxide Synthase Signaling Regulates K-Ras Plasma Membrane Interactions via Cyclic GMP-Dependent Protein Kinase 2.
Authors:
Journal:Mol Cell Biol
PubMed ID:27697864
Two novel atypical PKC inhibitors; ACPD and DNDA effectively mitigate cell proliferation and epithelial to mesenchymal transition of metastatic melanoma while inducing apoptosis.
Authors:
Journal:Int J Oncol
PubMed ID:29048609
Phosphatidylethanolamines modified by γ-ketoaldehyde (γKA) induce endoplasmic reticulum stress and endothelial activation.
Authors:
Journal:J Biol Chem
PubMed ID:21454544
Oncogenic PKC-ι activates Vimentin during epithelial-mesenchymal transition in melanoma; a study based on PKC-ι and PKC-ζ specific inhibitors.
Authors:
Journal:Cell Adh Migr
PubMed ID:29781749