SCF (C-Kit Ligand) Recombinant Human Protein
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引用和文献 (10)
Gibco™

SCF (C-Kit Ligand) Recombinant Human Protein

Recombinant Human Stem Cell Factor (SCF) is a bioactive protein intended for use in cell culture applications. SCF is a了解更多信息
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货号数量
CTP21131 mg
货号 CTP2113
价格（CNY)
-
数量:
1 mg
Recombinant Human Stem Cell Factor (SCF) is a bioactive protein intended for use in cell culture applications. SCF is a pleiotropic factor that acts in utero in germ cell and neural cell development and hematopoiesis. These activities are all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. The lyophilized, E. coli-expressed SCF Recombinant Human is verified for activity through dose-dependent proliferation of human MO-7e cells.

• Highly Pure—minimizes interference from other proteins or contaminants
• Active—get more results with less protein

High Purity
To help verify that GIBCO™ Recombinant growth factors are of the highest quality, each protein is analyzed for purity, along with structural homogeneity, to help ensure a biologically active protein. The purity of each GIBCO™ recombinant growth factor is verified by SDS-PAGE to be over 95% and to have endotoxin levels below 0.1 ng/μg.

High Biological Activity
All GIBCO™ Recombinant growth factors are verified for activity. Each lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our SCF Recombinant Human growth factor is determined by the dose-dependent proliferation of human MO-7e cells to have an ED50 range of 2–10 ng/mL (specific activity: 5 × 105–1 × 105 units/mg). Optimal concentration for individual applications should be determined by a dose response assay.

Related Links
• Our Full Collection of GIBCO™ Growth Factors
For Research Use Only. Not for use in diagnostic procedures.
规格
活动2 ng/ml
生物检测Proliferation of MO7e cells
分类Carrier-Free
内毒素水平<0.1 ng/μg
表达系统E. coli
基因别名SCF (c-kit Ligand)
分子量18.5 kDa
产品线Gibco
蛋白质家族Growth Factors & Receptors
蛋白质形式Full Length, Recombinant, Ligand
蛋白质长度164 aa
蛋白质子类型Other Proteins
纯化方法Sequential Chromatography
纯度或质量等级95 %
数量1 mg
研究类别Clinical Research, Stem Cell Research, Differentiation, Oncology
运输条件Wet Ice
来源Human
适用于（应用）Cell Culture
形式Lyophilized
重组Recombinant
种属Human
Unit Size1 mg
内容与储存
2°C to 8°C

常见问题解答 (FAQ)

我收到的装重组蛋白的小管看上去是空的。这正常吗？

我们的重组蛋白是以冻干粉的形式运送的。在大多数情况下管中可见蛋白粉末沉淀，有些时候粉末沉淀会脱离原位，粘附在管盖或管壁上。短暂的离心就可将蛋白粉末沉淀收集于管底。不过，并非所有的冻干蛋白均为肉眼可见。在大多数情况下，可见的白色粉末沉淀是原始缓冲溶液中的盐分，管中粉末沉淀大小与重组蛋白的量之间可能并无直接的相关性。如果重组蛋白在冻干前使用了不含盐分的溶剂，则可能并不容易看到粉末沉淀（通常为透明的）。您可通过在SDS PAGE上电泳少量的重溶蛋白溶液来确定蛋白的存在。一般来说，只需在丙烯酰胺胶中上样10ng蛋白，就可清晰地观察到确定预期大小的蛋白条带。

为何我在实验中未检测到重组蛋白的活性？

分析时间至关重要。每一种分析都需要进行条件优化并在响应的峰值时间进行。不同种类的细胞对于生长因子或细胞因子的响应可能会有差异。我们建议推荐用户按照产品手册中的推荐方法，使用相同的指示细胞重复我们的质控（QC）分析，来观察是否能够获得类似的反应结果。此外，血清也可能掩盖细胞的响应情况。对某些类型分析实验可能需要对细胞进行血清饥饿处理。

为何我的重组蛋白没有像产品材料中声称的活性？

如果您按照我们产品插页中的描述进行了测试，但未见到任何反应，则可能由以下几种原因导致：

- 蛋白未按照说明书推荐方式进行溶解。
- 溶解后的蛋白放置时间过长或蛋白发生沉淀。我们推荐您在重新溶解后的3-6个月内使用这些蛋白产品。
- 当重新溶解的蛋白溶液浓度低于0.1 mg/mL时，未添加载体蛋白。低于0.1 mg/mL的工作液应立即使用；我们不推荐长期储存此浓度的蛋白溶液。
- 蛋白溶液经过多次冻融循环或暴露于高温条件下。
- 使用错误类型的容器操作蛋白（某些蛋白非常容易粘附于某些塑料器皿上）。

为何我的重组蛋白不溶解？

溶解性问题可能由操作不当，或未使用推荐溶剂所导致。我们推荐您在打开试剂管前将冻干粉平衡至室温，并使用产品手册中推荐的缓冲液溶解蛋白产品（某些蛋白在低pH缓冲体系中溶解得更好）。请勿配制使用蛋白浓度大于1 mg/mL的浓度溶解蛋白产品。请勿剧烈震荡或混合蛋白溶液。将重新溶解的蛋白置于4°C条件下孵育过夜可能有助于解决任何可溶性问题。

如何确定重组蛋白的生物学活性？

生物分析法常被用来检测某一特定生长因子或细胞因子的生物学活性。在大多数情况下，研究人员一般基于各类指示性细胞——如某些原代细胞或细胞系——来开展生物分析。最为常用的生物分析法包括细胞增殖分析(https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Cell-Proliferation-Assay.pdf)，细胞趋化性分析(https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Chemotaxis-Assay.pdf)，细胞因子生成分析(https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Cytokine-Production-Assay.pdf)和细胞毒性分析(https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Cytotoxicity-Assay.pdf)。通常以ED50来表示某一细胞因子的生物活性，该值代表了能够诱发50%最大效应的细胞因子浓度。这一表示效价的方法仅适用于那些剂量效应曲线为S形的细胞因子。

View all SCF (C-Kit Ligand) Recombinant Human Protein FAQs

引用和文献 (10)

引用和文献
Abstract
PAF-acetylhydrolase expressed during megakaryocyte differentiation inactivates PAF-like lipids.
Authors:Foulks JM, Marathe GK, Michetti N, Stafforini DM, Zimmerman GA, McIntyre TM, Weyrich AS
Journal:Blood
PubMed ID:19395675
'Platelet activating factor (PAF) and PAF-like lipids induce inflammatory responses in target cells. These lipid mediators are inactivated by PAF-acetylhydrolase (PAF-AH). The PAF signaling system affects the growth of hematopoietic CD34(+) cells, but roles for PAF-AH in this process are unknown. Here, we investigated PAF-AH function during megakaryopoiesis and found ... More
Correlations of cell surface antigens with individual differences in radiosensitivity in human hematopoietic stem/progenitor cells.
Authors:Takahashi K, Monzen S, Hayashi N, Kashiwakura I
Journal:Radiat Res
PubMed ID:20095850
'To characterize the differences in the radiosensitivity of individual populations of human hematopoietic stem/progenitor cells (HSPCs), we examined the relationship among cell surface antigens, clonogenic potential and radiation survival. The expressions of CD34, CD38, CD45RA, CD110 and Tie-2, early differentiation pathway-related antigens in hematopoiesis, were analyzed on the surface of ... More
Radiosensitivity of human haematopoietic stem/progenitor cells.
Authors:Kato K, Omori A, Kashiwakura I
Journal:J Radiol Prot
PubMed ID:23295782
'The haematopoietic system is regenerative tissue with a high proliferative potential; therefore, haematopoietic stem cells (HSCs) are sensitive to extracellular oxidative stress caused by radiation and chemotherapeutic agents. An understanding of this issue can help predict haematopoietic recovery from radiation exposure as well as the extent of radiation damage to ... More
Immobilization, stabilization, and activation of human stem cell factor (SCF) on fragmin/protamine microparticle (F/P MP)-coated plates.
Authors:Kishimoto S, Oonuma F, Nakamura S, Hattori H, Nakamura S, Mori Y, Tanaka Y, Harada Y, Tagawa M, Ishihara M
Journal:J Biomed Mater Res B Appl Biomater
PubMed ID:19637375
'Fragmin (low-molecular-weight heparin)/protamine microparticles (F/P MPs) immobilize to culture plates, thereby retaining the binding of heparin-binding cytokines such as human stem cell factor (SCF). The purpose of this study was to evaluate the ability of F/P MP-coating to immobilize, stabilize, and enhance SCF-activity. Cell assays showed that SCF and preimmobilized ... More
The HIF-1 transcription complex is essential for translational control of myeloid hematopoietic cell function by maintaining mTOR phosphorylation.
Authors:Yasinska IM, Gibbs BF, Lall GS, Sumbayev VV
Journal:
PubMed ID:23872956
Mammalian myeloid cells are crucial effectors of host innate immune defense. Normal and pathological responses of these cells require adaptation to signaling stress through the hypoxia-inducible factor 1 (HIF-1) transcription complex. Adapted cells activate the mammalian target of rapamycin (mTOR), via S2448 phosphorylation, which induces de novo translation of vital ... More
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