二氢乙啶（氢乙啶）
二氢乙啶（氢乙啶）
Invitrogen™

二氢乙啶（氢乙啶）

The superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's了解更多信息
Have Questions?
货号数量
D231071 mL，5 mM 溶液（稳定溶于 DMSO）
货号 D23107
价格（CNY)
3,802.00
Online Exclusive
Ends: 31-Dec-2025
4,986.00
共减 1,184.00 (24%)
Each
添加至购物车
数量:
1 mL，5 mM 溶液（稳定溶于 DMSO）
价格（CNY)
3,802.00
Online Exclusive
Ends: 31-Dec-2025
4,986.00
共减 1,184.00 (24%)
Each
添加至购物车
The superoxide indicator dihydroethdium, also called hydroethidine, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red.
仅供科研使用。不可用于诊断程序。
规格
测试数量1 mL
数量1 mL，5 mM 溶液（稳定溶于 DMSO）
激发/发射518/606 nm
产品类型ROS Indicator
Unit SizeEach

常见问题解答 (FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.