Search
引用和文献 (188)
Invitrogen™
DiO'; DiOC18(3)（3,3'-双十八烷基氧杂碳菁氯酸盐）
发绿色荧光的亲脂性碳氰酸酯 DiOC18(3) 广泛用作亲脂性示踪剂。其在水中发弱荧光，但在掺入膜后会发出十分明亮且光稳定的荧光。在脂质环境中，其具有超高的消光系数和较短激发态寿命（∼1 纳秒）。添加到细胞中后，这种染料就会在细胞膜内侧向扩散。我们建议在二甲基甲酰胺了解更多信息
| 货号 | 数量 |
|---|---|
| D275 | 100 mg |
货号 D275
价格（CNY)
5,806.00
Each
数量:
100 mg
价格（CNY)
5,806.00
Each
发绿色荧光的亲脂性碳氰酸酯 DiOC18(3) 广泛用作亲脂性示踪剂。其在水中发弱荧光，但在掺入膜后会发出十分明亮且光稳定的荧光。在脂质环境中，其具有超高的消光系数和较短激发态寿命（∼1 纳秒）。添加到细胞中后，这种染料就会在细胞膜内侧向扩散。我们建议在二甲基甲酰胺 (DMF)、二甲基亚砜 (DMSO) 或乙醇中制备亲脂性示踪剂储备液，浓度为 1-2.5 mg/mL。与乙醇相比，DMF 更适合用作 DiO 的溶剂。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色
检测方法荧光
发射501 nm
激发波长范围484 nm
适用于（设备）荧光显微镜
数量100 mg
运输条件室温
产品类型DiO DiOC18(3)
亚细胞定位细胞膜&脂质, Lipids
Unit SizeEach
内容与储存
室温避光储存。
常见问题解答 (FAQ)
我用DiI一类的亲脂性花青染料进行细胞染色，但是当我试图继续用抗体标记时信号却丢失了。 这是什么原因所致？
亲脂性示踪剂跨膜转运需要多久？FAST亲脂性染料能快多少？
我应该使用哪种亲脂性示踪剂（DiO、DiI、DiD等）？
我想标记两种细胞群体然后进行细胞融合实验，哪种试剂最适合来成像？
我想用核酸染料来追踪我的细胞，比如DAPI或者Hoechst染料。您有什么建议吗？
引用和文献 (188)
引用和文献
Abstract
Subzonal organization of olfactory sensory neurons projecting to distinct glomeruli within the mouse olfactory bulb.
Journal:J Comp Neurol
PubMed ID:12619077
'Olfactory sensory neurons located in the nasal neuroepithelium send their axons directly into the olfactory bulb, where they contact the dendrites of second-order neurons in specialized spherical structures called glomeruli; each sensory neuron projects to a single glomerulus. All neurons expressing the same odorant receptor gene are confined to distinct
Direct membrane protein-DNA interactions required early in nuclear envelope assembly.
Journal:J Cell Biol
PubMed ID:16717124
'Among the earliest events in postmitotic nuclear envelope (NE) assembly are the interactions between chromatin and the membranes that will fuse to form the NE. It has been proposed that interactions between integral NE proteins and chromatin proteins mediate initial membrane recruitment to chromatin. We show that several transmembrane NE
Antigen-induced translocation of PKC-theta to membrane rafts is required for T cell activation.
Journal:Nat Immunol
PubMed ID:11376344
'Protein kinase C-theta (PKC-theta) is essential for mature T cell activation; however, the mechanism by which it is recruited to the TCR signaling machinery is unknown. Here we show that T cell stimulation by antibodies or peptide-major histocompatibility complex (MHC) induces translocation of PKC-theta to membrane lipid rafts, which localize
Beta-very low density lipoprotein is sequestered in surface-connected tubules in mouse peritoneal macrophages.
Journal:J Cell Biol
PubMed ID:8253839
'beta-very low density lipoprotein (VLDL) is a large lipoprotein with multiple apoprotein E (apoE) molecules that bind to the LDL receptors on mouse macrophages. Even though they bind to the same receptor, the endocytic processing of beta-VLDL differs from low density lipoprotein (LDL). LDL is rapidly delivered to perinuclear lysosomes
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
Journal:J Cell Biol
PubMed ID:8034750
'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this