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引用和文献 (4)
Invitrogen™
EnzChek™ 磷脂酶 C 直接检测试剂盒
该试剂盒可在基于微孔板的生化检测中连续监测磷脂酰胆碱特异性磷脂酶 C (PLC) 活性。该检测使用了 PCL 选择性荧光底物，后者在裂解后会发出明亮的绿色荧光。EnzChek™ 磷脂酶 C了解更多信息
| 货号 | 数量 |
|---|---|
| E10215 | 1 Kit |
货号 E10215
价格（CNY)
4,555.00
Each
数量:
1 Kit
价格（CNY)
4,555.00
Each
该试剂盒可在基于微孔板的生化检测中连续监测磷脂酰胆碱特异性磷脂酶 C (PLC) 活性。该检测使用了 PCL 选择性荧光底物，后者在裂解后会发出明亮的绿色荧光。
EnzChek™ 磷脂酶 C 直接检测试剂盒为监测 PC-PLC 活性提供了一种简单可靠的方法。每个试剂盒都能提供足够2块96孔微孔板以 200 µL 反应体积进行检测的试剂。PC-PLC 在涉及细胞凋亡和细胞存活的多种细胞信号转导通路以及从癌症到 HIV1-7 的各种疾病中发挥着至关重要的作用。该测定试剂盒使用带有染料标记 sn-2 酰基链的甘油磷酸乙醇胺作为 PC-PLC 的底物。PC-PLC 在磷酸基之前对底物进行切割并释放出染料标记的二酰甘油，后者产生可连续测量的阳性荧光信号。反应产物的最大吸收和荧光发射波长分别为 509 nm 和 516 nm。采用来自蜡样芽孢杆菌的纯化酶，该测定试剂盒能够在室温条件下在一小时后检测到低至 10 ⁄mU/mL 的 PC-PLC 含量。该试剂盒已经过验证可用于表征 PC-PLC 抑制，因为它是一种直接测量方法，所以消除了在化合物筛选中引起假阳性的可能性。
EnzChek™ 磷脂酶 C 直接检测试剂盒为监测 PC-PLC 活性提供了一种简单可靠的方法。每个试剂盒都能提供足够2块96孔微孔板以 200 µL 反应体积进行检测的试剂。PC-PLC 在涉及细胞凋亡和细胞存活的多种细胞信号转导通路以及从癌症到 HIV1-7 的各种疾病中发挥着至关重要的作用。该测定试剂盒使用带有染料标记 sn-2 酰基链的甘油磷酸乙醇胺作为 PC-PLC 的底物。PC-PLC 在磷酸基之前对底物进行切割并释放出染料标记的二酰甘油，后者产生可连续测量的阳性荧光信号。反应产物的最大吸收和荧光发射波长分别为 509 nm 和 516 nm。采用来自蜡样芽孢杆菌的纯化酶，该测定试剂盒能够在室温条件下在一小时后检测到低至 10 ⁄mU/mL 的 PC-PLC 含量。该试剂盒已经过验证可用于表征 PC-PLC 抑制，因为它是一种直接测量方法，所以消除了在化合物筛选中引起假阳性的可能性。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型BODIPY™ FL
过滤器类型FITC（荧光素）滤光片
数量1 Kit
运输条件室温
底物属性基于脂质的底物、化学底物
底物类型磷脂酶底物
目标酶磷脂酰胆碱特异性磷脂酶 C
颜色绿色
发射509⁄516
适用于（应用）磷脂酶 C 测定试剂盒
适用于（设备）微孔板读数仪
产品线EnzChek、Molecular Probes
产品类型磷脂酶检测
Unit SizeEach
内容与储存
I 试剂盒。在 -<20° 下避光储存于干燥处
常见问题解答 (FAQ)
What is the excitation/emission maxima of the reaction product when using the EnzChek Direct Phospholipase C Assay kit?
引用和文献 (4)
引用和文献
Abstract
Membrane Chaperone SecDF Plays a Role in the Secretion of Listeria monocytogenes Major Virulence Factors.
Journal:
PubMed ID:24056100
'Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocation system is the primary mediator of this secretion.
Lipid peroxidation is essential for phospholipase C activity and the inositol-trisphosphate-related Ca2+ signal.
Journal:
PubMed ID:24198393
'Reactive oxygen species (ROS) are produced in enzymatic and non-enzymatic reactions and have important roles in cell signalling but also detrimental effects. ROS-induced damage has been implicated in a number of neurological diseases; however, antioxidant therapies targeting brain diseases have been unsuccessful. Such failure might be related to inhibition of
Short- and Long-Term Regulation of Intestinal Na+/H+ Exchange Activity Associated with TLR2 Receptor Activation Is Independent of Nuclear Factor-?B Signaling.
Journal:
PubMed ID:23845891
'Type 2 Toll-like receptors (TLR2s) are expressed in cell membranes and recognize a wide range of pathogen-associated molecular patterns derived from bacteria, such as lipoteichoic acid (LTA). The aim of this study was to evaluate the effect of TLR2 activation by LTA on the activity of type 1 Na(+)/H(+) exchanger
Lipid-modifying enzymes in human tear fluid and corneal epithelial stress response.
Journal:
PubMed ID:24302584
Since homeostasis at the ocular surface requires a delicate balance between numerous factors, and the external environment contributes as an unpredictable component, we aimed to understand the role that various lipids and their regulators have in the complex process that maintains a healthy corneal surface. Through basic proteomics, we tested