EnzChek™ 磷脂酶 A2 检测试剂盒

引用和文献 (3)

Invitrogen™

EnzChek™ 磷脂酶 A2 检测试剂盒

该试剂盒采用我们针对磷脂酶 A2 (PLA2, A10072) 的独立检测方法，并结合必要的 Ez 和脂质以运行 2 块完整的 96了解更多信息

货号	数量
E10217	1 Kit

货号 E10217

价格（CNY)

5,586.00

添加至购物车

1 Kit

价格（CNY)

5,586.00

添加至购物车

该试剂盒采用我们针对磷脂酶 A2 (PLA2, A10072) 的独立检测方法，并结合必要的 Ez 和脂质以运行 2 块完整的 96 孔微孔板检测，可用于监测纯化酶制剂和细胞裂解物中的酶活性。EnzChek 磷脂酶 A2 试剂盒提供了足够 2 块 96 孔板以 200 μl 反应体积进行检测的试剂，可以进行 PLA2 的持续荧光监测。本产品可作为 (bis-BODIPY® FL C11-PC) 试剂 (B7701) 的替代品，通过提供 PLA2 选择性底物以及比率式检测方法，因此降低了仪器和检测条件产生的变化。磷脂酶 A2 或 PLA2 代表着用于水解磷脂的 sn-2 酯键的酶家族。这些酶的活性在心血管疾病、炎性疾病、神经系统疾病以及癌症中起着重要作用。EnzChek® 磷脂酶 A2 底物可实现对 PLA2 酶活性的灵敏、连续、快速实时监测。这种独特的底物对 PLA2 具有选择性，并且可用于基于强度或比率的检测模式。在基于强度的检测模式中，PLA2 活性可通过在大约 515 nm 单波长处的荧光强度增加进行监测。比率式分析基于该底物在切割前和切割后的明显荧光共振能量转移 (FRET) 发射，激发波长为 ≈ 460 nm，通过在 515/575 nm 波长处的发射强度比率变化检测 PLA2。两种检测模式均为低背景并且对 PLA2 具有高灵敏度和高特异性的简单方法。

仅供科研使用。不可用于诊断程序。

检测方法荧光强度

染料类型BODIPY™ FL

过滤器类型FITC（荧光素）滤光片

数量1 Kit

运输条件室温

底物属性基于脂质的底物、化学底物

底物类型磷脂酶底物

目标酶磷脂酶

颜色混合物，绿色

发射比率式：460-490⁄515，575，基于强度：460-490⁄515

适用于（应用）磷脂酶 A2 测定试剂盒

适用于（设备）微孔板读数仪

产品线EnzChek、Molecular Probes

产品类型磷脂酶检测

Unit SizeEach

内容与储存

1 套。储存于 -<20° 下的避光干燥处

常见问题解答 (FAQ)

What is the LoD for the EnzChek Phospholipase A2 Assay Kit (Cat. No. E10217)?

The EnzChek Phospholipase A2 Assay Kit (Cat. No. E10217) can detect PLA2 at 0.05 U/mL or lower.

The FRET-based substrate Red/Green BODIPY PC-A2 shows no change in fluorescence upon enzymatic cleavage. I'm not seeing an increasing green signal with higher concentrations of phospholipase A2 and no change in red fluorescence. Why is this?

There are numerous factors that can interfere with FRET-based detection. Here are a few items to consider:

a) Is there any component in the reaction solution that can quench green fluorescence?
b) Is there any component in the reaction solution that autofluoresces in the green or red emission range?
c) Too high of a substrate concentration may result in diffusional FRET: Lower the final working concentration of the substrate.
d) Ensure adequate dispersal of the substrate during the reaction. BODIPY dyes are very hydrophobic, and aggregates of the green product, BODIPY FL, can form an excimer and emit in the red range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

You provide Phospholipase A2 from honey bee venom in the EnzChek Phospholipase A2 Assay Kit. Can the kit be used with phospholipase A2 from other species?

Yes, phospholipase A2 from other species may be used as long as the phospholipase A2 can hydrolyze the sn-2 ester linkage of phospholipids and the enzymatic reaction can be done within a pH of >3 and <9.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (3)

引用和文献

Abstract

Crystal structure of the predicted phospholipase LYPLAL1 reveals unexpected functional plasticity despite close relationship to acyl protein thioesterases.

Authors:Bürger M, Zimmermann TJ, Kondoh Y, Stege P, Watanabe N, Osada H, Waldmann H, Vetter IR,

Journal:J Lipid Res

PubMed ID:22052940

'Sequence homology indicates the existence of three human cytosolic acyl protein thioesterases, including APT1 that is known to depalmitoylate H- and N-Ras. One of them is the lysophospholipase-like 1 (LYPLAL1) protein that on the one hand is predicted to be closely related to APT1 but on the other hand might ... More

Alterations in cerebrospinal fluid glycerophospholipids and phospholipase A2 activity in Alzheimer's disease.

Authors:Fonteh AN, Chiang J, Cipolla M, Hale J, Diallo F, Chirino A, Arakaki X, Harrington MG,

Journal:

PubMed ID:23868911

'Our aim is to study selected cerebrospinal fluid (CSF) glycerophospholipids (GP) that are important in brain pathophysiology. We recruited cognitively healthy (CH), minimally cognitively impaired (MCI), and late onset Alzheimer''s disease (LOAD) study participants and collected their CSF. After fractionation into nanometer particles (NP) and supernatant fluids (SF), we studied ... More

Group V secretory phospholipase A2 plays a pathogenic role in myocardial ischaemia-reperfusion injury.

Authors:Yano T, Fujioka D, Saito Y, Kobayashi T, Nakamura T, Obata JE, Kawabata K, Watanabe K, Watanabe Y, Mishina H, Tamaru S, Kugiyama K,

Journal:Cardiovasc Res

PubMed ID:21169294

Group V secretory phospholipase A(2) (sPLA(2)-V) is highly expressed in the heart. This study examined (i) the role of sPLA(2)-V in myocardial ischaemia-reperfusion (I/R) injury and (ii) the cooperative action of sPLA(2)-V and cytosolic PLA(2) (cPLA(2)) in myocardial I/R injury, using sPLA(2)-V knockout (sPLA(2)V(-/-)) mice. Myocardial I/R injury was created ... More