A facile method for immunofluorescence microscopy of highly autofluorescent human retinal sections using nanoparticles with large Stokes shifts.
Authors:Petty HR, Elner VM, Kawaji T, Clark A, Thompson D, Yang DL,
Journal:J Neurosci Methods
PubMed ID:20619292
'The human retina is rich in autofluorescent species, such as lipofuscin and melanin. Consequently, it is difficult to localize antigens in the human retina using immunofluorescence microscopy. To address this issue, we have developed a methodology to tag retinal antigens using quantum dot nanoparticles that absorb in the ultraviolet and ... More