内源性生物素封闭试剂盒
内源性生物素封闭试剂盒
Invitrogen™

内源性生物素封闭试剂盒

内源性生物素封闭试剂盒为预处理提供了一种方法和试剂,当使用生物素-亲和素或生物素-链霉素亲和素检测系统识别细胞靶标时,可以减少或消除背景信号。内源性生物素封闭试剂盒提供:•改善的结果—信噪比增加有助于细胞靶标识别• 易于使用—无移液器滴瓶可提供速度和便利性• 快速—可在 1 小时内减少内源性背景• 灵活性—可使用该试剂盒帮助改善传统免疫组织化学技术、免疫细胞化学技术、RNA FISH了解更多信息
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货号数量
E213901 kit
货号 E21390
价格(CNY)
3,839.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
3,839.00
Each
添加至购物车
内源性生物素封闭试剂盒为预处理提供了一种方法和试剂,当使用生物素-亲和素或生物素-链霉素亲和素检测系统识别细胞靶标时,可以减少或消除背景信号。

内源性生物素封闭试剂盒提供:
•改善的结果—信噪比增加有助于细胞靶标识别
• 易于使用—无移液器滴瓶可提供速度和便利性
• 快速—可在 1 小时内减少内源性背景
• 灵活性—可使用该试剂盒帮助改善传统免疫组织化学技术、免疫细胞化学技术、RNA FISH 和 ELISA 程序的结果

生物素自然出现,在多种细胞类型的细胞溶质和线粒体中作为酶辅因子发挥作用。内源性生物素封闭试剂盒通过两步封闭策略,尽可能减少了哺乳动物细胞和组织酶中内源性生物素的干扰。首先,向标本中加入过量的未标记链霉素亲和素以与富含内源性生物素的酶结合。随后,链霉素亲和素被过量的未标记生物素所封闭,从而有效地使细胞或组织样品不存在可用的生物素结合位点。
仅供科研使用。不可用于诊断程序。
规格
数量1 kit
试剂类型封闭⁄背景抑制试剂
运输条件室温
类型内源性生物素阻断试剂盒
Unit SizeEach
内容与储存
在冷藏冰箱(2°C 至 8°C)中储存。

引用和文献 (4)

引用和文献
Abstract
A facile method for immunofluorescence microscopy of highly autofluorescent human retinal sections using nanoparticles with large Stokes shifts.
Authors:Petty HR, Elner VM, Kawaji T, Clark A, Thompson D, Yang DL,
Journal:J Neurosci Methods
PubMed ID:20619292
'The human retina is rich in autofluorescent species, such as lipofuscin and melanin. Consequently, it is difficult to localize antigens in the human retina using immunofluorescence microscopy. To address this issue, we have developed a methodology to tag retinal antigens using quantum dot nanoparticles that absorb in the ultraviolet and ... More
Prevention of nonspecific binding of avidin.
Authors:Duhamel RC, Whitehead JS
Journal:Methods Enzymol
PubMed ID:2388571
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
Authors:Wood GS, Warnke R
Journal:J Histochem Cytochem
PubMed ID:7028859
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed ... More
Simultaneous inhibition of endogenous avidin-binding activity and peroxidase applicable for the avidin-biotin system using monoclonal antibodies.
Authors:Matsumoto Y
Journal:Histochemistry
PubMed ID:2415495
The use of the avidin-biotin technique in immunoperoxidase staining provides a simple and highly sensitive method for detecting the localization of antigens defined by monoclonal antibodies. However, endogenous biotin, which is widely distributed in tissues, often causes non-specific staining by binding to avidin [endogenous avidin-binding activity (EABA)]. Endogenous peroxidase activity ... More