MPO is inhibited by azide, diclofenac, methimazole, quercetin, rutin, and salicylhydroxamic acid. Make certain that samples do not include sodium azide. Endogenous catalases can interfere with the assay; catalase activity may be inhibited by using 3-amino-1,2,4-triazole. Detergents, common components in cell lysis buffers, should be avoided; use freeze/thawing and/or mechanical methods to lyse cells.
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