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引用和文献 (75)
Invitrogen™
EnzChek™ 磷酸盐检测试剂盒
EnzChek™ 磷酸盐检测试剂盒提供了一种快速且灵敏的分光光度法，可用于定量测定溶液中的无机磷酸盐以及在 ATP 酶或 GTP 酶反应过程中释放的磷酸盐。查看我们完整的荧光微孔板检测产品线。•灵敏度为 2-150 µM了解更多信息
| 货号 | 数量 |
|---|---|
| E6646 | 100 Assays |
货号 E6646
价格（CNY)
6,341.00
Each
数量:
100 Assays
价格（CNY)
6,341.00
Each
EnzChek™ 磷酸盐检测试剂盒提供了一种快速且灵敏的分光光度法，可用于定量测定溶液中的无机磷酸盐以及在 ATP 酶或 GTP 酶反应过程中释放的磷酸盐。
查看我们完整的荧光微孔板检测产品线。
•灵敏度为 2-150 µM 磷酸盐
• 基于微孔板的分光光度法可以检测最大吸光度从 330 nm 至 360 nm 的变化
• 持续监测由 ATP 酶或 GTP 酶活性生成的磷酸盐
在存在无机磷酸盐的情况下，2-氨基-6-巯基-7-甲基嘌呤核糖核苷 (MESG) 底物被嘌呤核苷磷酸化酶 (PNP) 转化为核糖 1-磷酸和 2-氨基-6-巯基-7-甲基嘌呤产物。MESG 的这种酶促转化会导致使用分光光度法测量到最大吸光度的波长从底物的 330 nm 变为产物的 360 nm。
测定试剂盒的灵敏度在 2-150 µM 无机磷酸盐（1 mL 体积中 2-150 纳摩尔磷酸盐）范围内，反应可在 6.5-8.5 的 pH 值范围内进行。EnzChek™ 磷酸盐反应足够快速且为定量测定，可用于停流动力学实验。
查看我们完整的荧光微孔板检测产品线。
•灵敏度为 2-150 µM 磷酸盐
• 基于微孔板的分光光度法可以检测最大吸光度从 330 nm 至 360 nm 的变化
• 持续监测由 ATP 酶或 GTP 酶活性生成的磷酸盐
在存在无机磷酸盐的情况下，2-氨基-6-巯基-7-甲基嘌呤核糖核苷 (MESG) 底物被嘌呤核苷磷酸化酶 (PNP) 转化为核糖 1-磷酸和 2-氨基-6-巯基-7-甲基嘌呤产物。MESG 的这种酶促转化会导致使用分光光度法测量到最大吸光度的波长从底物的 330 nm 变为产物的 360 nm。
测定试剂盒的灵敏度在 2-150 µM 无机磷酸盐（1 mL 体积中 2-150 纳摩尔磷酸盐）范围内，反应可在 6.5-8.5 的 pH 值范围内进行。EnzChek™ 磷酸盐反应足够快速且为定量测定，可用于停流动力学实验。
仅供科研使用。不可用于诊断程序。
规格
检测方法比色法
染料类型MESG
产品规格管
数量100 Assays
运输条件室温
颜色紫外线
适用于（应用）磷酸盐检测
适用于（设备）分光光度计
产品线EnzChek
产品类型磷酸盐检测
Unit SizeEach
内容与储存
在冰箱中（-5°C 至 -30°C）储存。
引用和文献 (75)
引用和文献
Abstract
Regulation of vascular smooth muscle tone by N-terminal region of caldesmon. Possible role of tethering actin to myosin.
Journal:J Biol Chem
PubMed ID:10652307
'To assess the functional significance of tethering actin to myosin by caldesmon in the regulation of smooth muscle contraction, we investigated the effects of synthetic peptides, containing the myosin-binding sequences in the N-terminal region of caldesmon, on force directly recorded from single permeabilized smooth muscle cells of ferret portal vein.
Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae.
Journal:Biochemistry
PubMed ID:11305920
'CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacteriol. 181, 2001]. In the work described here,
The role of Mg2+ cofactor in the guanine nucleotide exchange and GTP hydrolysis reactions of Rho family GTP-binding proteins.
Journal:J Biol Chem
PubMed ID:10843989
'The biological activities of Rho family GTPases are controlled by their guanine nucleotide binding states in cells. Here we have investigated the role of Mg(2+) cofactor in the guanine nucleotide binding and hydrolysis processes of the Rho family members, Cdc42, Rac1, and RhoA. Differing from Ras and Rab proteins, which
The antibacterial peptide pyrrhocoricin inhibits the ATPase actions of DnaK and prevents chaperone-assisted protein folding.
Journal:Biochemistry
PubMed ID:11258915
'Recently, we documented that the short, proline-rich antibacterial peptides pyrrhocoricin, drosocin, and apidaecin interact with the bacterial heat shock protein DnaK, and peptide binding to DnaK can be correlated with antimicrobial activity. In the current report we studied the mechanism of action of these peptides and their binding sites to
The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase: sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates.
Journal:Proc Natl Acad Sci U S A
PubMed ID:10688898
'Enterobactin, the tris-(N-(2,3-dihydroxybenzoyl)serine) trilactone siderophore of Escherichia coli, is synthesized by a three-protein (EntE, B, F) six-module nonribosomal peptide synthetase (NRPS). In this work, the 142-kDa four-domain protein EntF was bisected into two double-domain fragments: a 108-kDa condensation and adenylation construct, EntF C-A, and a 37-kDa peptidyl carrier protein (PCP)