Novex™ TBE-Urea Gels, 6%
Novex™ TBE-Urea Gels, 6%
Actual product may vary
Invitrogen™

Novex™ TBE-Urea Gels, 6%

变性聚丙烯酰胺凝胶，可将单链 DNA 寡核苷酸或 RNA 分解成明显、清晰的条带
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货号孔
EC68652BOX12孔
EC6865BOX10孔
EC68655BOX15孔
货号 EC68652BOX
价格（CNY)
2,137.00
Each
添加至购物车
孔:
12孔
请求批量或定制报价
价格（CNY)
2,137.00
Each
添加至购物车
Novex™ TBE-Urea Gels are denaturing polyacrylamide gels that resolve single-stranded DNA oligos or RNA into sharp, distinct bands. These 6% gels are optimized for the analysis and purification of products ranging from 25–110 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and northern blot analysis.

Novex TBE-Urea Gels can be stained by silver staining, ethidium bromide, and SYBR™ Green staining techniques after electrophoresis. The compact gels are available in 6%, 10%, or 15% formats and are designed to run on the XCell SureLock™ Mini-Cell.

Advantages of TBE-Urea Gels:
• Contains 7M urea for maximum denaturation
• Excellent resolution for fast size and purity confirmations of DNA or RNA oligos
• High resolution of short single-strand oligonucleotides
• Requires ∼10% sample concentration and volume of large or agarose gels
• Accurate and reproducible results

Formulation
Novex TBE-Urea gels are made with high-purity reagents, including Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, APS, and 7M urea. Strict quality control ensures that gels and buffers are DNase- and RNase-free.

Recommended Buffers
For optimum performance, Novex™ TBE-Urea sample buffers and Novex™ TBE Running Buffer are strongly recommended for use with these gels. For analytical applications, Novex TBE-Urea Sample Buffer is recommended contains urea, the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

For Research Use Only. Not for use in diagnostic procedures.
规格
描述Novex TBE-Urea Gels, 6%, 12-well
产品类型TBE 凝胶
数量10 块凝胶/盒
样品类型Single-stranded DNA Oligos/RNA
运输条件湿冰
适用于（设备）XCell SureLock Mini-Cell
凝胶百分比6%
凝胶类型TBE-Urea
分离范围20 至 800 bp
孔12孔
Unit SizeEach
内容与储存
• 货号是指一盒10块凝胶
• Novex™ TBE 尿素凝胶的有效期为4至8周，具体取决于凝胶类型

在 4°C 下储存。

常见问题解答 (FAQ)

我进行TBE-尿素凝胶电泳时出现了高背景和拖尾条带。我应该如何才能解决这一问题？

这里有一些对您实验的建议：

•RNA样本可能被RNA酶降解了。遵循标准注意事项以避免污染。
•确保在上样前对样本在70°C加热3分钟。如果没有这样做，则寡核苷酸不会被完全变性，导致出现拖尾。
•确保在上样前彻底将尿素冲洗出上样孔。尿素会持续从凝胶渗入上样孔内。尿素很浓而且会使样本形成球状。
•上样体积超过10 µL可能引起拖尾。这一体积比琼脂糖凝胶所用的上样体积小。
•如果没有使用超纯水（18毫欧姆），则可能出现拖尾条带和高背景。
•检查电泳缓冲液和上样缓冲液是否正确制备和稀释。
•确保使溴酚蓝染料迁移到达边缘。过早停止电泳可能导致电泳效果较差。

Invitrogen TBE-尿素系统可以使用含有福尔马林的上样缓冲液吗？

该系统可以使用多种上样缓冲液配方；但是，我们发现根据上样缓冲液的不同，电泳条带的形态会有很大的不同。在评估了尿素、福尔马林以及各种缓冲液系统之后，我们发现使用尿素、Ficoll和TBE缓冲溶液能获得最清晰、最整齐的条带。使用含福尔马林的上样缓冲液将得到模糊、不清晰的条带。

我可以对TBE凝胶或TBE-尿素凝胶染色吗？如何染色？

可以，对于EB染色，将凝胶在超纯水配制的2 µg/mL EB溶液中浸泡20分钟。接下来使用超纯水进行3次洗涤脱色，每次10分钟。在紫外光下观察条带。

I am getting high background and smeared bands on my TBE-urea gels. What should I do?

Here are some suggestions for your experiments:

- The RNA samples may have been degraded by RNases. Use standard precautions to prevent contamination.
- Make sure samples are heated for 3 minutes at 70°C just prior to loading. If this is not done, the oligonucleotides will not be fully denatured, which may result in a smeared background.
- Be sure to vigorously flush urea out of sample wells just prior to loading the sample. Urea will continually seep from the gel into the well. Urea is very dense and will force the sample into a ball.
- A sample volume over 10 µL may result in smearing. This volume is less than may be used with agarose gels.
- If ultrapure water (18 milliohms) is not used, smeared bands and high background may result.
- Check to make sure the running and sample buffers have been prepared and diluted correctly.
- Make sure to run the bromophenol blue until it reaches the slot. Stopping the run short can result in less than optimal results.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use a sample buffer containing formamide with the Invitrogen TBE-urea system?

There are many sample buffer formulations used; however, we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide provided fuzzy, indistinct bands.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

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